In vitro assessment of gastrointestinal viability of two photosynthetic bacteria, Rhodopseudomonas palustris and Rhodobacter sphaeroides.

被引：37
作者：
Zhou X.X. [1 ]
Pan Y.J. [1 ]
Wang Y.B. [1 ]
Li W.F. [1 ]
机构：
[1] Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou
来源：
Journal of Zhejiang University SCIENCE B | 2007年 / 8卷 / 9期
基金：
中国国家自然科学基金;
关键词：
Photosynthetic bacteria; Probiotics; Primary culture; Intestinal epithelial cell; S91;
D O I：
10.1631/jzus.2007.B0686
中图分类号：
学科分类号：
摘要：
The objectives of this study were to assess the potential of two photosynthetic bacteria (PSB), Rhodopseudomonas palustris HZ0301 and Rhodobacter sphaeroides HZ0302, as probiotics in aquaculture. The viability of HZ0301 and HZ0302 in simulated gastric transit conditions (pH 2.0, pH 3.0 and pH 4.0 gastric juices) and in simulated small intestinal transit conditions (pH 8.0, with or without 0.3% bile salts) was tested. The effects of HZ0301 and HZ0302 on the viability and permeability of intestinal epithelial cell in primary culture of tilapias, Oreochromis nilotica, were also detected. All the treatments were determined with three replicates. The simulated gastric transit tolerance of HZ0301 and HZ0302 strains was pH-dependent and correspondingly showed lower viability at pH 2.0 after 180 min compared with pH 3.0 and pH 4.0. Both HZ0301 and HZ0302 were tolerant to simulated small intestine transit with or without bile salts in our research. Moreover, there was no significant difference (P>0.05) among three treatments including the control and the groups treated with HZ0301 or HZ0302 both in intestinal epithelial cell viability and membrane permeability, showing no cell damage. In summary, this study demonstrated that HZ0301 and HZ0302 had high capacity of upper gastrointestinal transit tolerance and were relatively safe for intestinal epithelial cells of tilapias.
引用
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页码：686 / 692
页数：6
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