A study on the potential interaction between cyclodextrin and lipoxygenase

To get a more complete view on the lipoxygenase (LOX) catalysis in presence of cyclodextrin, the investigation into the interaction between cyclodextrins (CDs) and LOX was carried out. Effects of cyclodextrins on the activity and structure of LOX were explored in this work. It is confirmed that inhibition effect induced by complexation of CDs and LOX plays a leading role in inhibition factors of LOX catalysis in presence of CDs. Inhibition of β-cyclodextrin on LOX depended on concentration and tended to be intensified with the increase of β-CD. The enhancement of intrinsic fluorescence of LOX induced by β-CD was detected, which was probably due to the formation of complexes between aromatic amino acid residues of LOX and β-CD. The results of circular dichroism assay indicated that β-CD altered the secondary structure and microenvironment of LOX which was responsible for inhibition of enzyme catalysis.