Novel [l,2,4]triazolo[3,4-a]isoquinoline chalcones as new chemotherapeutic agents: Block IAP tyrosine kinase domain and induce both intrinsic and extrinsic pathways of apoptosis

被引：0
作者：
Magda F. Mohamed
Farid M. Sroor
Nada S. Ibrahim
Ghada S. Salem
Hadeer H. El-Sayed
Marwa M. Mahmoud
Menna-Allah M. Wagdy
Amina M. Ahmed
Aya-Allah T. Mahmoud
Somia S. Ibrahim
Mariam M. Ismail
Sanaa Mohy Eldin
Fatma M. Saleh
Hamdi M. Hassaneen
Ismail A. Abdelhamid
机构：
[1] Cairo University,Department of Chemistry (Biochemistry Branch), Faculty of Science
[2] University of Jeddah,Department of Chemistry, Faculty of Science and Arts, Khulais
[3] National Research Centre,Organometallic and Organometalloid Chemistry Department
[4] Cairo University,Chemistry Department (Biotechnology
[5] National Research Centre,Biomolecular Chemistry Program), Faculty of Science
[6] Cairo University,Department of Pesticide Chemistry
来源：
Investigational New Drugs | 2021年 / 39卷
关键词：
[l,2,4]Triazolo[3,4-; ]isoquinoline; Chalcones; Cytotoxicity; IAP-Caspase3; Cytochrome c oxidase; Morphological characterization;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Two novel chemotherapeutic chalcones were synthesized and their structures were confirmed by different spectral tools. Theoretical studies such as molecular modeling were done to detect the mechanism of action of these compounds. In vitro cytotoxicity showed a strong effect against all tested cell lines (MCF7, A459, HepG2, and HCT116), and low toxic effect against normal human melanocytes (HFB4). The lung carcinoma cell line was chosen for further molecular studies. Real-time PCR demonstrated that the two compounds upregulated gene expression of (BAX, p53, casp-3, casp-8, casp-9) genes and decreased the expression of anti-apoptotic genes bcl2, CDK4, and MMP1. Flow-cytometry indicated that cell cycle arrest of A459 was induced at the G2/M phase and the apoptotic percentage increased significantly compared to the control sample. Cytochrome c oxidase and VEGF enzyme activity were detected by ELISA assay. SEM tool was used to follow the morphological changes that occurred on the cell surface, cell granulation, and average roughness of the cell surface. The change in the number and morphology of mitochondria, cell shrinkage, increase in the number of cytoplasmic organelles, membrane blebbing, chromatin condensation, and apoptotic bodies were observed using TEM. The obtained data suggested that new chalcones exerted their pathways on lung carcinoma through induction of two pathways of apoptosis.
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页码：98 / 110
页数：12
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