Identification of autofluorescent cells in human angioimmunoblastic T-cell lymphoma

被引:0
作者
Igor Buchwalow
Dmitri Atiakshin
Vera Samoilova
Werner Boecker
Markus Tiemann
机构
[1] Institute for Hematopathology,Research Institute of Experimental Biology and Medicine
[2] Burdenko Voronezh State Medical University,undefined
来源
Histochemistry and Cell Biology | 2018年 / 149卷
关键词
Autofluorescence; Angioimmunoblastic T-cell lymphoma; Leucocytes; Eosinophils;
D O I
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中图分类号
学科分类号
摘要
Endogenous cell autofluorescence is a common nuisance that complicates the use of fluorescence microscopy. When using fluorescence-labeled antibodies for specific cell labeling in tissue sections of human angioimmunoblastic T-cell lymphoma (AITL), we encountered with a problematic autofluorescence of multiple cells. These cells emitted fluorescence signals in the green, red and deep-red spectral range. Characterization of these autofluorescent cells solely on the basis of their autofluorescence failed. To identify these enigmatic cells residing the lymphoma tissue, we combined two imaging techniques—fluorescence and brightfield microscopy. Combined fluorescence/brightfield imaging of cells immunolabeled with a panel of CD antibodies raised against diverse cellular components allowed us to identify the autofluorescent cells in the AITL as eosinophils. These cells tended to accumulate in the vicinity of capillaries and arterioles apparently mediating the process of angiogenesis resembling other angiogenesis-associated diseases.
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页码:169 / 177
页数:8
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