An echographic image processing method has been developed, and validated by in vitro experiments, for the 3D reconstruction of the long bones of the newborn. The reconstruction of successive parallel cross-sections is obtained by a 2D reconstruction technique using radial B-scan image processing. The automatic segmentation of all the calculated images allows the extraction of the external contours of the skeleton. After structuring the explored volume using a contour association method, a contour interpolation step is required to solve the anisotropy problem, to obtain a 3D representation with cubic voxel lists. The results are encouraging, and a new mechanical part prototype of the acquisition system is under test for in vivo experiments. The main originality of the paper lies in the combination of different steps to obtain a practical solution to a clinical problem.