A glassy carbon electrode modified with molecularly imprinted poly(aniline boronic acid) coated onto carbon nanotubes for potentiometric sensing of sialic acid

被引：0
作者：
Fuhui Huang
Bengao Zhu
Haochen Zhang
Yue Gao
Chunmei Ding
Hong Tan
Jianshu Li
机构：
[1] Sichuan University,College of Polymer Science and Engineering
[2] Sichuan University,State Key Laboratory of Polymer Materials Engineering
来源：
Microchimica Acta | 2019年 / 186卷
关键词：
Sialic acid; Poly(aniline boronic acid); Carbon nanotubes; Physiological pH value; Molecularly imprinted polymer; Potentiometric response; Electrochemical sensor;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A potentiometric sensor for sialic acid (SA) was developed based on molecular imprinting technique. The sensor was fabricated by modifying carbon nanotubes (CNT) and an SA-imprinted poly(aniline boronic acid) (PABA) film on a glassy carbon electrode (GCE). The detection strategy capitalizes on the change of electrochemical potential resulting from boronic acid-SA interaction. The imprinted PABA combines the functions of SA-binding boronic acid groups and the imprinting effect, thus endowing it with both chemical and sterical recognition capability. The imprint factor (IF, compared to a non-molecularly imprinted polymer) is 1.74. The sensor can well differentiate SA from its analogs at physiological pH values and has a linear potentiometric response (R2 = 0.998) in 80 μM to 8.2 mM SA concentrations range with a detection limit of 60 μM (at S/N = 3). The sensor was applied to the determination of SA in serum samples and gave recoveries between 93% and 105%.
引用
收藏
相关论文
共 136 条
  • [11] Numnuam A(2002)Potentiometric saccharide detection based on the pK(a) changes of poly(aniline boronic acid) J Am Chem Soc 124 12486-12493
  • [12] Limbut W(2010)A reusable interface constructed by 3-aminophenylboronic acid-functionalized multiwalled carbon nanotubes for cell capture, release, and cytosensing Adv Funct Mater 20 992-999
  • [13] Kanatharana P(2009)Noninvasive sialic acid detection at cell membrane by using phenylboronic acid modified self-assembled monolayer gold electrode J Am Chem Soc 131 12022-12023
  • [14] Thavarungkul P(2017)Sensitive determination of sialic acid expression on living cells by using an ITO electrode modified with graphene, gold nanoparticles and thionine for triple signal amplification Microchim Acta 184 3841-3850
  • [15] Matsuno K(1959)Polyol complexes and structure of the benzeneboronate ion J Org Chem 24 769-774
  • [16] Suzuki S(2003)Saccharide imprinting of poly(aniline boronic acid) in the presence of fluoride Analyst 128 803-806
  • [17] Tebani A(2003)Anomalous binding profile of phenylboronic acid with N-acetylneuraminic acid (Neu5Ac) in aqueous solution with varying pH J Am Chem Soc 125 3493-3502
  • [18] Schlemmer D(2010)Assessment of tumor metastasis by the direct determination of cell-membrane sialic acid expression Angew Chem Int Ed 49 5494-5497
  • [19] Imbard A(2015)Surface-enhanced Raman scattering imaging of cancer cells and tissues via sialic acid-imprinted nanotags Chem Commun 51 17696-17699
  • [20] Rigal O(2017)Preparation of sialic acid-imprinted fluorescent conjugated nanoparticles and their application for targeted cancer cell imaging ACS Appl Mater Interfaces 9 3006-3015
12345678910