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Cloning of two β-xylanase-encoding genes from Aspergillus niger and their expression in Saccharomyces cerevisiae
被引：0
作者：
M. Luttig
I.S. Pretorius
W.H. van Zyl
机构：
[1] University of Stellenbosch,Department of Microbiology
来源：
Biotechnology Letters
|
1997年
/
19卷
关键词：
Xylanase Activity;
Xylanase Gene;
Feruloyl Esterase;
Xylanolytic Enzyme;
ADH2 Promoter;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Two endo-β-1,4-xylanase-encoding genes were amplified from Aspergillus niger ATCC 90196 mRNA, inserted between the yeast ADH2 promoter and terminator sequences (genes designated XYN4 and XYN5) and expressed in Saccharomyces cerevisiae. The nucleotide sequences of the XYN4 and XYN5 genes revealed that both genes encode 211-amino acid proteins that are 92% identical to each other. Both the Xyn4 and Xyn5 enzymes have pH and temperature optima of pH 4 and 60°C, respectively. Autoselective S. cerevisiae strains were developed that allowed β-xylanase production and secretion in complex medium.
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页码：411 / 415
页数：4
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