Targeted base editing in the plastid genome of Arabidopsis thaliana

被引：0
作者：
Issei Nakazato
Miki Okuno
Hiroshi Yamamoto
Yoshiko Tamura
Takehiko Itoh
Toshiharu Shikanai
Hideki Takanashi
Nobuhiro Tsutsumi
Shin-ichi Arimura
机构：
[1] The University of Tokyo,Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences
[2] Tokyo Institute of Technology,School of Life Science and Technology
[3] Kurume University School of Medicine,Division of Microbiology, Department of Infectious Medicine
[4] Kyoto University,Department of Botany, Graduate School of Science
来源：
Nature Plants | 2021年 / 7卷
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摘要：
Bacterial cytidine deaminase fused to the DNA binding domains of transcription activator-like effector nucleases was recently reported to transiently substitute a targeted C to a T in mitochondrial DNA of mammalian cultured cells1. We applied this system to targeted base editing in the Arabidopsis thaliana plastid genome. The targeted Cs were homoplasmically substituted to Ts in some plantlets of the T1 generation and the mutations were inherited by their offspring independently of their nuclear-introduced vectors.
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页码：906 / 913
页数：7
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