Cell Type-Specific Transcriptome Analysis of the Soybean Leaf Paraveinal Mesophyll Layer

被引:0
作者
Siau Sie Voo
Howard Dean Grimes
Bernd Markus Lange
机构
[1] Washington State University,Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory
[2] Washington State University,School of Molecular Biosciences
来源
Plant Molecular Biology Reporter | 2013年 / 31卷
关键词
Acid phosphatase; Paraveinal mesophyll; Sucrose facilitator; Transcriptome; Vegetative storage protein; Vegetative lipoxygenase;
D O I
暂无
中图分类号
学科分类号
摘要
The paraveinal mesophyll (PVM) layer of soybean leaves, which contains cells with various unique ultrastructural properties, has been studied for decades, and several hypotheses regarding its functional role have been developed. Here, we describe a method for obtaining PVM cells using laser capture microdissection and pressure catapulting, subsequent isolation of RNA from these cells, and downstream microarray analysis. A cell type-specific transcriptome analysis was used to compare the gene expression patterns in PVM cells with those of a mesophyll cell type (palisade parenchyma) as a reference. Transcripts related to vegetative storage protein (Vsp) and certain vegetative lipoxygenase (Vlx) isoforms were significantly enriched in PVM cells, which is in accordance with prior work that demonstrated an accumulation of the corresponding proteins. Potential roles of Vsp and Vlx in phosphate mobilization and defense responses, respectively, are discussed. In addition, we found an enrichment of several transport-related genes in PVM cells. Building on our transcriptome data, we provide a fresh discussion of the hypothesis that the PVM plays a role in photoassimilate mobilization and translocation.
引用
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页码:210 / 221
页数:11
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共 48 条
[31]   Cerebellar 'transcriptome' reveals cell-type and stage-specific expression during postnatal development and tumorigenesis [J].
Schuller, Ulnch ;
Kho, Alvin T. ;
Zhao, Qing ;
Ma, Qiufu ;
Rowitch, David H. .
MOLECULAR AND CELLULAR NEUROSCIENCE, 2006, 33 (03) :247-259
[32]   Quantitative In-Depth Analysis of the Mouse Mast Cell Transcriptome Reveals Organ-Specific Mast Cell Heterogeneity [J].
Akula, Srinivas ;
Paivandy, Aida ;
Fu, Zhirong ;
Thorpe, Michael ;
Pejler, Gunnar ;
Hellman, Lars .
CELLS, 2020, 9 (01)
[33]   Single Cell Transcriptome Analysis of Niemann-Pick Disease, Type C1 Cerebella [J].
Cougnoux, Antony ;
Yerger, Julia C. ;
Fellmeth, Mason ;
Serra-Vinardell, Jenny ;
Martin, Kyle ;
Navid, Fatemeh ;
Iben, James ;
Wassif, Christopher A. ;
Cawley, Niamh X. ;
Porter, Forbes D. .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (15) :1-23
[34]   Single-cell allele-specific expression analysis reveals dynamic and cell-type-specific regulatory effects [J].
Qi, Guanghao ;
Strober, Benjamin J. ;
Popp, Joshua M. ;
Keener, Rebecca ;
Ji, Hongkai ;
Battle, Alexis .
NATURE COMMUNICATIONS, 2023, 14 (01)
[35]   Luminal and Glandular Epithelial Cells from the Porcine Endometrium maintain Cell Type-Specific Marker Gene Expression in Air-Liquid Interface Culture [J].
Schmidhauser, Meret ;
Ulbrich, Susanne E. ;
Schoen, Jennifer .
STEM CELL REVIEWS AND REPORTS, 2022, 18 (08) :2928-2938
[36]   Comprehensive expression analysis with cell-type-specific transcriptome in ALS-linked mutant SOD1 mice: Revisiting the active role of glial cells in disease [J].
Yamashita, Hirofumi ;
Komine, Okiru ;
Fujimori-Tonou, Noriko ;
Yamanaka, Koji .
FRONTIERS IN CELLULAR NEUROSCIENCE, 2023, 16
[37]   Transcriptome analysis with different leaf blades identifies the phloem-specific phosphate transporter OsPHO1;3 required for phosphate homeostasis in rice [J].
Yan, Meng ;
Xie, Mengyang ;
Chen, Wang ;
Si, Wen-jing ;
Lin, Hong-hui ;
Yang, Jian .
PLANT JOURNAL, 2024, 118 (03) :905-919
[38]   Single-cell RNA-seq analysis reveals ploidy-dependent and cell-specific transcriptome changes in Arabidopsis female gametophytes [J].
Song, Qingxin ;
Ando, Atsumi ;
Jiang, Ning ;
Ikeda, Yoko ;
Chen, Z. Jeffrey .
GENOME BIOLOGY, 2020, 21 (01)
[39]   Single-cell RNA-seq analysis reveals ploidy-dependent and cell-specific transcriptome changes in Arabidopsis female gametophytes [J].
Qingxin Song ;
Atsumi Ando ;
Ning Jiang ;
Yoko Ikeda ;
Z. Jeffrey Chen .
Genome Biology, 21
[40]   Transcriptome analysis reveals molecular signature and cell-type difference of Homo sapiens endothelial-to-mesenchymal transition [J].
Bronson, Ronald ;
Lyu, Junfang ;
Xiong, Jianhua .
G3-GENES GENOMES GENETICS, 2023, 13 (12)