Organisation and molecular analysis of repeated DNA sequences in the rice blast fungus Magnaporthe grisea

被引:0
|
作者
Pradeep Kachroo
Munish Ahuja
Sally A. Leong
B. B. Chattoo
机构
[1] Department of Microbiology and Biotechnology Centre,
[2] M.S. University of Baroda,undefined
[3] Baroda-390 002,undefined
[4] India,undefined
[5] Department of Plant Pathology and USDA-ARS Plant Disease Research Unit,undefined
[6] 1630 Linden Drive,undefined
[7] University of Wisconsin,undefined
[8] Madison,undefined
[9] WI-53706,undefined
[10] USA,undefined
来源
Current Genetics | 1997年 / 31卷
关键词
Key words Repeated DNA; Rice blast; Fingerprinting; Transposon;
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中图分类号
学科分类号
摘要
The distribution of a previously described repeated DNA sequence present as a 1.3-kb PstI fragment in the genome of the rice blast fungus Magnaporthe grisea was analysed by carrying out DNA fingerprint analysis of 36 isolates including rice, non-rice and laboratory strains. The analysis of various higher-molecular-weight PstI fragments with homology to the 1.3-kb repeat revealed that these may arise predominantly from transposon insertions or point mutations. Analysis of a 5.1-kb derivative revealed both a point mutation at a PstI site and an insertion of a putative transposable element which caused an increase in molecular weight from 1.3 to 5.1 kb. Another repeat element of 1.4 kb was identified and found to exist in association with the 1.3-kb repeat. Both 1.3- and 1.4-kb elements were found to be parts of MGR583 (Hamer et al. 1989), a LINE-like element. These elements were present in a high copy number in all the rice and a majority of non-rice pathogens indicating that MGR583 is not a host-specific sequence as reported earlier. Our results suggest that repeated DNA elements in M. grisea have amplified independently of one another and further indicate that different isolates of M. grisea may have evolved from several distinct lines of origin.
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页码:361 / 369
页数:8
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