Quercetin, rutin, and their combinations modulate penile phosphodiesterase-5′, arginase, acetylcholinesterase, and angiotensin-I-converting enzyme activities: a comparative study

被引：12
作者：
Adefegha S.A. [1 ]
Oboh G. [1 ]
Fakunle B. [1 ]
Oyeleye S.I. [1 ,2 ]
Olasehinde T.A. [3 ,4 ]
机构：
[1] Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology Akure, PMB 704, Akure, Ondo State
[2] Department of Biomedical Technology, Federal University of Technology Akure, PMB 704, Akure, Ondo State
[3] Department of Biochemistry and Microbiology, University of Fort Hare, Alice, 5700, Eastern Cape
[4] Nutrition and Toxicology Division, Food Technology Department, Federal Institute of Industrial Research, Oshodi, PMB 21023, Lagos
来源：
Comparative Clinical Pathology | 2018年 / 27卷 / 3期
关键词：
Erectile dysfunction; Phosphodiesterase-5′; Quercetin; Rutin;
D O I：
10.1007/s00580-018-2664-y
中图分类号：
学科分类号：
摘要：
This study demonstrates the effects of rutin and quercetin and their various combinations on phosphodiesterase-5 (PDE-5), arginase, acetylcholinesterase (AChE), and angiotensin-I-converting enzyme (ACE) activities in vitro. The effects of the flavonoids against Fe2+- and sodium nitroprusside (SNP)-induced lipid peroxidation in rats’ corpus cavernosum tissues were also investigated. Quercetin and rutin were dissolved in dimethylsulfoxide (DMSO) to a final concentration of 1 mM each. Thereafter, their combinations (50% quercetin + 50% rutin [Q1:R1]; 75% quercetin + 25% rutin [Q3:R1]; 25% quercetin + 75% rutin [Q1:R3]) were prepared. Our findings revealed that both flavonoids inhibited PDE-5, arginase, AChE, and ACE activities. Rutin exhibited significantly higher inhibitory effects on PDE-5, AChE, and ACE activities compared to quercetin. Considering the combinations, Q1:R3 was more potent compared to Q1:R1 and Q3:R1. Both flavonoids inhibited Fe2+- and SNP-induced lipid peroxidation in rat’s corpus cavernosa tissues. Rutin also showed higher inhibitory effects on Fe- and SNP-induced lipid peroxidation. Similarly, the combinatorial effects of the flavonoids revealed that Q1:R3 significantly inhibited malondialdehyde (MDA) production compared to Q1:R1 and Q3:R1. In conclusion, our findings suggest that the combination of quercetin and rutin is more potent than their individual effect. © 2018, Springer-Verlag London Ltd., part of Springer Nature.
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页码：773 / 780
页数：7
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