Microsatellite multiplex panels for population genetic analysis of red sea bream Pagrus major

被引：0
作者：
Enrique Blanco Gonzalez
Masaki Aritaki
Nobuhiko Taniguchi
机构：
[1] The Research Institute of Marine Bioresources of Fukuyama University,Coastal Fisheries and Aquaculture Division, Seikai National Fisheries Research Institute
[2] Fisheries Research Agency,undefined
来源：
Fisheries Science | 2012年 / 78卷
关键词：
Multiplex; Microsatellite; Red sea bream; Stock enhancement; Genetic diversity; Population structure;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this study, multiplex PCR panels were developed to amplify 20 microsatellite markers for red sea bream, Pagrus major, with the aim of reducing labor and material costs associated with genetic analysis of this species. The usefulness of these panels was validated in 200 fish collected at five sampling locations. The occurrence of null alleles was suggested at five markers, which were dropped from further analysis. The remaining 15 microsatellite loci showed high levels of heterozygosity (HE range 0.34–0.97, HO range 0.32–1) and a wide range in the number of alleles per locus (A; range 5–46). Increasing the number of microsatellite loci from three to ten and 15 improved the performance of the panels for population differentiation (FST) and estimation of Nei’s (DS) genetic distance. The results of this study confirm the usefulness of genotyping a large number of microsatellite DNA markers to expand our knowledge of red sea bream population genetics.
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页码：603 / 611
页数：8
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