An examination of the ability of titanium dioxide nanoparticles and its conjugates with oligonucleotides to penetrate into eucariotis cells

被引：5

作者：

Zarytova V.F. ^{[1
]}

Zinov'ev V.V. ^{[2
]}

Ismagilov Z.R. ^{[3
]}

Levina A.S. ^{[1
]}

Repkova M.N. ^{[1
]}

Shikina N.V. ^{[3
]}

Evdokimov A.A. ^{[2
]}

Belanov E.F. ^{[2
]}

Balakhnin S.M. ^{[2
]}

Serova O.A. ^{[2
]}

Baiborodin S.I. ^{[4
]}

Malygin E.G. ^{[2
]}

Zagrebel'nyi S.N. ^{[4
]}

机构：

[1] Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090

[2] The Vektor State Research Center of Virology and Biotechnology, Federal Service for Supervision in the Sphere of Protecting Consumer Rights and Human Wellbeing, Kol'tsovo, Novosibirsk oblast 630559, ABK

[3] Institute of Catalysis, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090

[4] State Educational Institution of Higher Professional Education, Novosibirsk State University Rosobrazovaniya, Russian Federation, Novosibirsk 630090

来源：

Nanotechnologies in Russia | 2009年 / 4卷 / 9-10期

关键词：

Actin Filament; TiO2 Nanoparticles; Herceptin; Titanium Dioxide NANOPARTICLES; MDBK Cell;

D O I：

10.1134/S1995078009090158

中图分类号：

学科分类号：

摘要：

In this study we examine the possibility that TiO2 nanoparticles and their conjugates can penetrate into cultivated cells without any special transfection procedures. Oligonucleotides and their derivates were conjugated with the TiO2 nanoparticles, which were obtained as colloidal solutions at a concentration of TiO2 0.3M by TiCl4 hydrolysis. The electronic microscopy of various cell cultures (KCT, Vero, and MDCK) treated with nanoparticle solutions (20 μg/μl) showed that nanoparticles could enter the cells and accumulate in the vacuoles and phagosomes and form inclusions in cytoplasm. Thus, we demonstrated the penetration of TiO2 nanoparticles and their oligonucleotide conjugates into intracellular space without any auxiliary operations. Most other researches used electroporation techniques for similar purposes [1, 2, 5]. © 2009 Pleiades Publishing, Ltd.

引用

页码：732 / 735

页数：3

共 10 条

[1] Paunesku T., Vogt S., Lai B., Maser J., Stojicevic N., Thurn K.T., Osipo C., Liu H., Legnini D., Wang Z., Lee C., Woloschak G.E., Intracellular Distribution of TiO2-DNA Oligonucleotide Nanoconjugates Directed to Nucleolus and Mitochondria Indicates Sequence Specificity, Nano Lett., 7, 3, pp. 596-601, (2007)
[2] Endres R.J., Raunesku T., Vogt S., Meade T.J., Woloschak G.E., DNA-TiO2 Nanoconjugates Labeled with Magnetic Resonance Contrast Agents, J. Am. Chem. Soc., 129, pp. 15760-15761, (2007)
[3] Gavrilov V.Y., Zenkovets G.A., Influence of Conditions Providing Precipitation of Titanium Dioxide Hydrogel on the Porous Xerogel Structure, Kinet. Katal., 31, 1, pp. 168-173, (1990)
[4] Levina A., Pyshnaya I., Repkova M., Rar V., Zarytova V., Oligonucleotide Probes Containing Polylysine Residues for Fabrication of DNA Chips on Various Solid Surfaces, Biotechnol. J., 7, pp. 879-885, (2007)
[5] Paunesku T., Rajh T., Wiederrecht G., Maser J., Vogt S., Stojicevic N., Protic M., Lai B., Oryhon J., Thurnauer M., Woloschak G., Biology of TiO2-Oligonucleotide Nanocomposites, Nat. Mater., 2, pp. 343-346, (2003)
[6] Rajh T., Chen L.X., Lukas K., Liu T., Thurnauer M.C., Tiede D.M., Surface Restructuring of Nanoparticles: An Efficient Route for Ligand-Metal Oxide Crosstalk, J. Phys. Chem. B, 106, pp. 10543-10552, (2002)
[7] Plenat F., Klein-Monhoven N., Marie B., Vignaud J.M., Duprez A., Cell and Tissue Distribution of Synthetic Oligonucleotides in Healthy and Tumor-Bearing Nude Mice: An Autoradiographic, Immunohistological, and Direct Fluorescence Microscopy Study, Am. J. Pathol., 147, 1, pp. 124-135, (1995)
[8] Jiang W., Kim B.Y.S., Rutka J.T., Chan W.C.W., Nanoparticle-Mediated Cellular Response Is Size-Dependent, Nat. Nanotechnol., 3, pp. 145-150, (2008)
[9] Zhang A.-P., Sun Y.-P., Photocatalytic Killing Effect of TiO2 Nanoparticles on Ls-174-t Human Colon Carcinoma Cells, World J. Gastroenterol., 10, 21, pp. 3191-3193, (2004)
[10] Houjin H., Pierstorff E., Osawa E., Ho D., Active Nanodiamond Hydrogels for Chemotherapeutic Delivery, Nano Lett., 7, 11, pp. 3305-3314, (2007)

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