Micrometer-resolution X-ray tomographic full-volume reconstruction of an intact post-mortem juvenile rat lung

被引:0
作者
Elena Borisova
Goran Lovric
Arttu Miettinen
Luca Fardin
Sam Bayat
Anders Larsson
Marco Stampanoni
Johannes C. Schittny
Christian M. Schlepütz
机构
[1] Paul Scherrer Institute,Swiss Light Source
[2] University of Bern,Institute of Anatomy
[3] École Polytechnique Fédérale de Lausanne,Center for Biomedical Imaging
[4] ETH Zurich,Institute for Biomedical Engineering
[5] European Synchrotron Radiation Facility,Department of Pulmonology and Clinical Physiology
[6] Grenoble University Hospital,Inserm UA7, Synchrotron Radiation for Biomedicine Laboratory (STROBE)
[7] University Grenoble Alpes,Department of Surgical Sciences
[8] Uppsala University,undefined
来源
Histochemistry and Cell Biology | 2021年 / 155卷
关键词
X-ray tomography; Fast tomography; Image reconstruction; Large volume tomography; Lung imaging;
D O I
暂无
中图分类号
学科分类号
摘要
In this article, we present an X-ray tomographic imaging method that is well suited for pulmonary disease studies in animal models to resolve the full pathway from gas intake to gas exchange. Current state-of-the-art synchrotron-based tomographic phase-contrast imaging methods allow for three-dimensional microscopic imaging data to be acquired non-destructively in scan times of the order of seconds with good soft tissue contrast. However, when studying multi-scale hierarchically structured objects, such as the mammalian lung, the overall sample size typically exceeds the field of view illuminated by the X-rays in a single scan and the necessity for achieving a high spatial resolution conflicts with the need to image the whole sample. Several image stitching and calibration techniques to achieve extended high-resolution fields of view have been reported, but those approaches tend to fail when imaging non-stable samples, thus precluding tomographic measurements of large biological samples, which are prone to degradation and motion during extended scan times. In this work, we demonstrate a full-volume three-dimensional reconstruction of an intact rat lung under immediate post-mortem conditions and at an isotropic voxel size of (2.75 µm)3. We present the methodology for collecting multiple local tomographies with 360° extended field of view scans followed by locally non-rigid volumetric stitching. Applied to the lung, it allows to resolve the entire pulmonary structure from the trachea down to the parenchyma in a single dataset. The complete dataset is available online (https://doi.org/10.16907/7eb141d3-11f1-47a6-9d0e-76f8832ed1b2).
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页码:215 / 226
页数:11
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