Toxic Effect of Zinc Ions Is Accompanied by Acidification of the Cytoplasm in Cultured Rat Cerebellar Granule Neurons

被引:0
作者
M. O. Shedenkova
E. V. Stelmashook
N. K. Isaev
机构
[1] Research Center of Neurology,
[2] M. V. Lomonosov Moscow State University,undefined
来源
Bulletin of Experimental Biology and Medicine | 2022年 / 173卷
关键词
survival; neurons; zinc ions; acidosis; рН;
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摘要
Addition of 0.07 mM zinc ions to the culture medium induced death of cerebellar granule neurons. This was preceded by a decrease in intracellular pH by 0.86±0.12 and an increase in the level of intracellular zinc registered by increasing fluorescence of FluoZin3. However, intracellular acidosis caused by acidification of the culture medium to pH 6.0 was not toxic to neurons, and even significantly increased their survival under the action of zinc ions. In general, the overload of the neurons cytoplasm with zinc ions causes acidification of the cytoplasm, which is probably associated with the activation of Zn2+/H+ exchangers and is a protective mechanism for the neurocytotoxic effect of zinc ions.
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页码:539 / 543
页数:4
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  • [1] Stelmashuk EV(2007)Effect of acidosis, oxidative stress, and glutamate toxicity on the survival of mature and immature cultured cerebellar granule cells Neurochem. J. 1 66-69
  • [2] Belyaeva EA(2017)Zinc as a neuromodulator in the central nervous system with a focus on the olfactory bulb Front. Cell. Neurosci. 11 297-578
  • [3] Isaev NK(2019)ZnT2 is an electroneutral proton-coupled vesicular antiporter displaying an apparent stoichiometry of two protons per zinc ion PLoS Comput. Biol. 15 565-1175
  • [4] Blakemore LJ(2012)Zinc networks: the cell-specific compartmentalization of zinc for specialized functions Biol. Chem. 393 1171-43
  • [5] Trombley PQ(2008)Role of acidosis, NMDA receptors, and acid-sensitive ion channel 1a (ASIC1a) in neuronal death induced by ischemia Biochemistry (Mosc). 73 9217-312
  • [6] Golan Y(2020)Fluorescein derivatives as fluorescent probes for pH monitoring along recent biological applications Int. J. Mol. Sci. 21 35-373
  • [7] Alhadeff R(2016)Intracellular zinc distribution in mitochondria, ER and the Golgi apparatus Int. J. Physiol. Pathophysiol. Pharmacol. 8 303-136
  • [8] Warshel A(2017)Zinc and cortical plasticity Neurotoxicity of zinc. Adv. Neurobiol. 18 347-791
  • [9] Assaraf YG(2009)Zinc at glutamatergic synapses Brain Res. Rev. 59 126-1663
  • [10] Hennigar SR(2009)Zinc in the physiology and pathology of the CNS Neuroscience. 158 780-1044