Reactivity of Ferrocenylcarbodiimide with DNA Duplex Containing Single-mismatched Base Pairs

被引:0
作者
Kosuke Mukumoto
Sadayoshi Watanabe
Takahiko Nojima
Michinori Waki
Shigeori Takenaka
机构
[1] Kyushu University,Department of Applied Chemistry, Faculty of Engineering
[2] Kyushu Institute of Technology,Department of Applied Chemistry, Faculty of Engineering
来源
Analytical Sciences | 2007年 / 23卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
Ferrocenylcarbodiimide (1), which is known to react with a guanine (G) or thymine (T) base of single stranded DNA, was allowed to react with DNA duplex having a single mismatched base pair of G-T, T-T, or T-cytosine (C). Electrophoreograms of the reaction mixture showed that 1 could react with G or T base of the mismatched sites on the DNA duplex. However, 1 also reacted with the G base of the terminal site on the DNA duplex. This showed that 1 can react with an unpaired base or unstable base pair such as a terminal or mismatched base on the DNA duplex. Electrochemical mismatch detection could be achieved after hybridization of the ferrocenylated mismatched DNA duplex with a selected DNA probe-immobilized electrode. These results revealed that 1 has a potentiality of serving as a labeling reagent of mismatched bases on the DNA duplex, which is important in the search for heterozygous single nucleotide polymorphisms (SNPs).
引用
收藏
页码:645 / 649
页数:4
相关论文
共 54 条
  • [1] Palecek E(1992)undefined Methods in Enzymology 212 139-undefined
  • [2] Novak D F(1986)undefined Proc. Natl. Acad. Sci. U. S. A. 83 586-undefined
  • [3] Casna N J(1991)undefined J. Biol. Chem. 266 12035-undefined
  • [4] Fischer S G(1991)undefined Am. J. Hum. Genet. 48 1186-undefined
  • [5] Ford J P(1991)undefined Proc. Natl. Acad. Sci. U. S. A. 88 5423-undefined
  • [6] Ganguly A(2005)undefined Tetrahedron 61 11705-undefined
  • [7] Baldwin C T(2007)undefined Anal. Sci. 23 115-undefined
  • [8] Strobel D(2000)undefined Clin. Sci. 99 569-undefined
  • [9] Conway D(2000)undefined Biochim. Biophys. Acta 1502 433-undefined
  • [10] Horton W(1999)undefined Clin. Chim. Acta 285 143-undefined