Determination of the molar extinction coefficients of the b800 and b850 absorption bands in light-harvesting complexes 2 derived from three purple photosynthetic bacteria rhodoblastus acidophilus, Rhodobacter sphaeroides, and Phaeospirillum molischianum by extraction of bacteriochlorophyll a

被引：16
作者：
Saga Y. [1 ,2 ]
Hirota K. [1 ]
机构：
[1] Department of Chemistry, Faculty of Science and Engineering, Kinki University, Higashi-Osaka, Osaka
[2] PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama
来源：
Analytical Sciences | 2016年 / 32卷 / 7期
关键词：
B800; B850; Bacteriochlorophyll; Molar extinction coefficient; Photosynthetic protein;
D O I：
10.2116/analsci.32.801
中图分类号：
学科分类号：
摘要：
The molar extinction coefficients of light-harvesting complex 2 (LH2) have been ambiguous in spite of its fame and wide utilization. Herein we determine the molar extinction coefficients of the LH2 proteins derived from the three purple photosynthetic bacteria Rhodoblastus acidophilus, Rhodobacter sphaeroides and Phaeospirillum molischianum at 298 K by direct extraction of bacteriochlorophyll (BChl) a from the lyophilized proteins, followed by estimation of BChl a amounts from their electronic absorption spectra. © The Japan Society for Analytical Chemistry.
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页码：801 / 804
页数：3
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