Cleistanthin B causes G1 arrest and induces apoptosis in mammalian cells

被引：0

作者：

Pradheep Kumar C.P. ^{[1
,3
]}

Pande G. ^{[2
]}

Shanmugam G. ^{[1
,3
]}

机构：

[1] Cancer Biology Division, School of Biological Sciences, Madurai Kamaraj University

[2] Ctr. for Cell. and Molecular Biology, Uppal Road

[3] School of Biological Sciences, Madurai Kamaraj University

来源：

Apoptosis | 1998年 / 3卷 / 6期

关键词：

Apoptosis; Cell cycle; Cleistanthin B; Etoposide;

D O I：

10.1023/A:1009658518998

中图分类号：

学科分类号：

摘要：

Cleistanthin B is a potential anticancer agent isolated from the tropical plant Cleistanthus collinus. We have previously shown that cleistanthin B is clastogenic and induces micronuclei formation and chromosomal aberrations.1 We now show that this compound inhibits DNA synthesis in Chinese hamster ovary (CHO) cells and induces apoptosis in cervical carcinoma (SiHa) cells. Flow cytometric analysis of cleistanthin treated CHO cells revealed that they were blocked in G1. Cervical carcinoma (SiHa) cells exposed to cleistanthin B shrank, rounded up and had condensed chromatin and fragmented nuclei. DNA isolated from cleistanthin treated cells exhibited the characteristic apoptotic ladder when electrophoresed in agarose gels. These results were confirmed by flow cytometry. Etoposide, a structurally similar compound also induced apoptosis in these cells although with a difference. Etoposide induced apoptosis after permitting cells to enter into S phase, while cleistanthin B stopped entry of cells into S phase and subsequently drove them to apoptosis.

引用

页码：413 / 419

页数：6

共 17 条

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