Effect of Ro 5-4864 and PK11195 on protein phosphorylation in mitochondria isolated from primary cultures of rat astrocytes

被引：0
作者：
Grachev D.E. [1 ]
Krestinina O.V. [2 ]
Baburina Y.L. [2 ]
Reiser G. [1 ]
Azarashvili T.S. [2 ]
机构：
[1] Institute of Neurobiochemistry, Otto-von-Guericke-University of Magdeburg, Medicine Faculty, Magdeburg 39120
[2] Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast 142290, Institutskaya ul.
来源：
Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology | 2010年 / 4卷 / 1期
关键词：
Astrocyte mitochondria; Permeability transition pore (PTP); PK11195; Protein phosphorylation; Ro; 5-4864; TSPO/PBR;
D O I：
10.1134/S1990747810010071
中图分类号：
学科分类号：
摘要：
Astrocytes are the most numerous cell type within the central nervous system. Earlier, high-affinity binding sites for [3H]PK 11195 and [3H]Ro 5-4864 with the properties of the peripheral-type benzodiazepine receptor were detected in primary cultures of astrocytes. TSPO/PBR was shown to be localized in mitochondria. Recently, we showed that TSPO/PBR ligands, Ro 5-4864 and PK11195, were able to modulate the function of non-specific pore (PTP) in brain and liver mitochondria as well as protein phosphorylation in the presence of threshold calcium concentrations. In the present study for the first time the function of astrocyte mitochondria were studied under condition of PTP opening. Parameters of PTP induction were measured by means of simultaneous registrations of the membrane potential, calcium accumulation and calcium release as well as detection of the oxygen consumption with selective electrodes. Four phosphorylated proteins in range of 67 kDa, 46 kDa, 48 kDa and 3.5 kDa have been found under these conditions. It was established that in astrocyte mitochondria TSPO/PBR exists in monomer form (18 kDa). The phosphorylation level of these proteins was found to be modulated by TSPO/PBR ligands, Ro 5-4864 and PK11195, in a range of concentrations from 0.01 to 1 μM, in the same way as it was earlier described for brain mitochondria [Azarashvili et al., J Neurochem., 2005]. © 2010 Pleiades Publishing, Ltd.
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页码：43 / 49
页数：6
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