Duplex PCR Assay for Identification of Tissue of Cattle and Buffalo Origin Targeting Mitochondrial Cytochrome b Gene

被引：5

作者：

Kumbhar V.H. ^{[1
,2
]}

Kumar R.R. ^{[2
]}

Mendiratta S.K. ^{[2
]}

Choudhary A. ^{[2
]}

Kumar D. ^{[2
]}

Kumari S. ^{[2
,3
]}

Rana P. ^{[2
,4
]}

机构：

[1] Division of Livestock Products Technology, COVAS, GADVASU, Ludhiana

[2] Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar, Bareilly

[3] Division of Livestock Products Technology, PGIVER, RAJUVAS, Jaipur

[4] Division of Livestock Products Technology, DUVASU, Mathura

来源：

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences | 2020年 / 90卷 / 2期

关键词：

Buffalo; Cattle; Cytochrome b gene; Duplex PCR assays; Meat species identification;

D O I：

10.1007/s40011-019-01102-z

中图分类号：

学科分类号：

摘要：

The aim of this study was to develop PCR assay for simultaneous detection of tissues from cattle and buffalo in a single PCR reaction. Species-specific primers for cattle and buffalo species were designed through homology comparisons and alignment of partial sequences of Cyt.b gene from common food animals. The conditions for species-specific PCR amplification were optimized in terms of quantity and concentration of various components for PCR mix and annealing temperature for each set of designed species-specific primer pairs. The assay revealed the amplification of genomic DNA from cattle to amplicon size of 655 bp, whereas DNA template from buffalo to amplicon length of 249 bp. Simultaneous amplification of DNA templates from cattle and buffalo was observed in duplex PCR assay. Thus, the developed assay could identify the tissues of cattle and buffalo origin, detecting even both the species together and being advantageous over species-specific PCR. © 2019, The National Academy of Sciences, India.

引用

页码：287 / 291

页数：4

共 20 条

[1]

Zhang C., Semi-nested multiplex PCR enhanced method sensitivity of species detection in further-processed meats, Food Control, 31, pp. 326-330, (2013)

[2]

Amaral J.S., Santos C.G., Melo V.S., Costa J., Oliveira M.B.P.P., Mafra I., Identification of duck, partridge, pheasant, quail, chicken and turkey meats by species-specific PCR assays to assess the authenticity of traditional game meat sausages, Food Control, 47, pp. 190-195, (2015)

[3]

Cheng X., He W., Huang F., Huang M., Zhou G., Multiplex real-time PCR for the identification and quantification of DNA from duck, pig and chicken in Chinese blood curds, Food Res Int, 60, pp. 30-37, (2014)

[4]

Kallea E., Kubista M., Rensing C., Multi-template polymerase chain reaction, Biomol Detect Quantif, 2, pp. 11-29, (2014)

[5]

Park Y.H., Uzzaman M.R., Park J.W., Kim S.W., Lee J.H., Kim K.S., Detection of meat origin (species) using polymerase chain reaction, Korean J Food Sci Anim Resour, 33, 6, pp. 696-700, (2013)

[6]

Ali M.E., Razzak M.A., Hamid S.B.A., Rahman M.M., Amin M.A., Rashid N.R.A., Multiplex PCR assay for the detection of five meat species forbidden in Islamic foods, Food Chem, 177, pp. 214-224, (2015)

[7]

Guan F., Jin Y.T., Zhao J., Xu A.C., Luo Y.Y., A PCR method that can be further developed into PCR-RFLP assay for eight animal species identification, J Anal Methods Chem, (2018)

[8]

Abbas O., Zadravec M., Baeten Mikus V.T., Lesic T., Vulic A., Pleadin J., Analytical methods used for the authentication of food of animal origin, Food Chem, 246, pp. 6-17, (2018)

[9]

Hanapi U.K., Desa M.N., Ismail A., Mustafa S., A higher sensitivity and efficiency of common primer multiplex PCR assay in identification of meat origin using NADH dehydrogenase subunit 4 gene, J Food Sci Technol, 52, 7, pp. 4166-4175, (2015)

[10]

Kumari R., Rank D.N., Kumar S., Joshi C.G., Lal S.V., Real time PCR- an approach to detect meat adulteration, Buffalo Bull, 34, 1, pp. 124-129, (2015)

← 1 2 →