A self-assembling RNA aptamer-based nanoparticle sensor for fluorometric detection of Neomycin B in milk

被引：0

作者：

Kai Ling

Hongyan Jiang

Linlin Zhang

Yang Li

Lu Yang

Chen Qiu

Fu-Rong Li

机构：

[1] Jinan University,The Second Clinical Medical College (Shenzhen People’s Hospital)

[2] Jinan University,School of Medicine

[3] Shenzhen Institute of Geriatrics,Institute of Biomedical Engineering, Key Laboratory of Biomedical Material of Tianjin

[4] Chinese Academy of Medical Sciences & Peking Union Medical College,Department of Nuclear Medicine, Xinhua Hospital, School of Medicine

[5] Shanghai Jiaotong University,undefined

来源：

Analytical and Bioanalytical Chemistry | 2016年 / 408卷

关键词：

Gold nanoparticle; RNA aptamer; Aptasensor; Self-assembly; Neomycin B;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

To date, there are few reports regarding the development of RNA aptamer-based biosensors for the detection of small molecules. The possible reason is attributed to the weak nuclease resistance of RNA in biological environments. In this study, we have developed an RNA aptamer-based gold nanoparticle (AuNP) sensor for fluorometric detection of Neomycin B in milk. This aptasensor depends on the self-assembly of the RNA aptamer/Neomycin B complex and fluorescence quenching by AuNPs. This biosensor exhibited a low detection limit of 0.01 μM, with a linear dynamic range from 0.1 to 10 μM in milk, and a good selectivity toward Neomycin B. Specifically, because of the shorter RNA fragments and the nuclease inhibition ability of the RNA-modified AuNPs, the RNA sequences remained stable during the experiments. This work will serve as an example for the development of novel biosensors based on RNA aptamers.

引用

页码：3593 / 3600

页数：7