Improved callus formation and plant regeneration for shed microspore culture in asparagus (Asparagus officinalis L.)

被引:0
作者
M. Peng
D. J. Wolyn
机构
[1] Department of Horticultural Science,
[2] University of Guelph,undefined
[3] Guelph,undefined
[4] Ontario,undefined
[5] N1G 2W1,undefined
[6] Canada Fax: +1-519-7670755 e-mail: dwolyn@evbhort.uoguelph.ca,undefined
来源
Plant Cell Reports | 1999年 / 18卷
关键词
Key words Asparagus; Shed microspore culture; Androgenesis; Cell division; Callus;
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学科分类号
摘要
 To establish an efficient asparagus microspore culture system, experiments were conducted to investigate the effects of medium components, period of cold pretreatment for flower buds, and period of anther co-culture on culture response. All factors affected the frequency of asparagus microspore division and the yields of microspore-derived calli. The best results were obtained by pretreating genotype G459 flower buds at 4  °C for 7–9 days, co-culturing anthers with shed microspores for 14 days, and including 6% sucrose, 2 mg l–1α-naphthaleneacetic acid and 1 mg l–1 N6-benzylaminopurine in the culture medium. After 4 days of culture, most shed microspores contained starch-like bodies and died. The 2% of shed microspores lacking these structures divided to produce microcalli. For the best treatments in the different experiments, about 140 calli per 100 anthers were recovered. Cultured on four different regeneration media, 19.6–21% and 3.9–8.0% of microspore-derived calli produced shoots and embryos, respectively, and ultimately plantlets, among which 49% were haploid, 34% diploid, 4% triploid and 11% tetraploid.
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页码:954 / 958
页数:4
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