Improved formation of regenerable callus in isolated microspore culture of maize: Impact of carbohydrates, plating density and time of transfer

被引：15
作者：
Nägeli M. [1 ]
Schmid J.E. [2 ]
Stamp P. [2 ]
Büter B. [2 ]
机构：
[1] Central Laboratory, Wankdorfstrasse 10
[2] Institute of Plant Sciences, Swiss Fed. Institute of Technology, Universitätstr. 2
来源：
Plant Cell Reports | 1999年 / 19卷 / 2期
关键词：
Androgenesis; Isolated microspores; Maltose; Plant regeneration; Zea mays L;
D O I：
10.1007/s002990050730
中图分类号：
学科分类号：
摘要：
Pure fractions of maize (Zea mays L.) microspores at various densities were exposed to defined media containing different concentrations of maltose and sucrose. In general, lower carbohydrate concentrations (60, 90 g/l) yielded higher frequencies of embryo-like structures than a high concentration (120g/l). Optimum cell density seemed to depend on the genotype, but densities above 80,000 microspores/ml led to reduced embryogenesis in all genotypes tested. Direct comparison of maltose and sucrose as carbohydrate source in the induction medium clearly demonstrated the superiority of maltose with regard to the regeneration frequency. For two out of three genotypes tested, maltose also enhanced the formation of embryo-like structures. The time of embryo transfer to callus induction media had a significant effect on regeneration frequency.
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页码：177 / 184
页数：7
相关论文
共 38 条
[1]  
Afele, J.C., Kannenberg, L.W., Keats, R., Sohota, S., Swanson, E.B., Increased induction of microspore embryos following manipulation of donor plant environment and culture temperature in corn (Zea mays L.) (1992) Plant Cell Tissue Organ Cult, 28, pp. 87-90
[2]  
Barloy, D., Beckert, M., Improvement of regeneration ability of androgenetic embryos by early anther transfer in maize (1993) Plant Cell Tissue Organ Cult, 33, pp. 45-50
[3]  
Batty, N., Dunwell, J., Effect of maltose on the response of potato anthers in culture (1989) Plant Cell Tissue Organ Cult, 18, pp. 221-226
[4]  
Beaumont, V., (1995) Anther Culture of Corn: Genetic Mapping and Improvement of the Tissue Culture Technique, , PhD thesis, Université Paris VII
[5]  
Büter, B., (1992) Einflussfaktoren der in Vitro Haploidinduktion bei Zea Mays L, , PhD thesis, Swiss Federal Institute of Technology (ETH), Zürich
[6]  
Büter, B., In vitro haploid production in maize (1997) In Vitro Production of Higher Plants, 4, pp. 37-71. , Jain SM, Sopory SK, Veilleuy RE (eds) Kluwer, Dordrecht
[7]  
Chu, C.C., The N6-medium and its applications to anther culture of cereal crops (1981) Proc Symp Plant Tissue Cult, pp. 43-50. , Bejing 1978. Pitman, Boston
[8]  
Deimling, S., Flehinghaus, T., Schneider, I., Geiger, H.H., Influence of post-plating temperature and carbohydrate source in maize and rye anther culture (1990) Abstracts 7th International Congress Plant Tissue Cell Culture, , Amsterdam, 1990
[9]  
Dieu, P., Beckert, M., Further studies of androgenetic embryo production and plant regeneration from in vitro cultured anthers in maize (Zea mays L.) (1986) Maydica, 31, pp. 245-259
[10]  
Dunwell, J.M., Anther culture in Nicotiana tabacum: The role of the culture vessel atmosphere in pollen embryo induction and growth (1979) J Exp Bot, 30, pp. 419-428
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