Nitric oxide counteracts the senescence of detached rice leaves induced by dehydration and polyethylene glycol but not by sorbitol

In the present study, we evaluated the protective effect of nitric oxide(NO) against senescence of rice leaves enhanced by water deficit. Dehydration(DH), polyethylene glycol (PEG) and sorbitol (ST) treatments were used toinducewater deficit. Senescence of rice leaves was determined by the decrease ofprotein content. NO donors[N-tert-butyl-α-phenylnitrone (PBN), sodiumnitroprusside (SNP), 3-morpholinosydnonimine (SIN-1), and ascorbic acid +NaNO2] were effective in inhibiting senescence of dehydrated andPEG-treated rice leaves, but had no effect on senescence of ST-treated riceleaves. PEG or DH resulted in a marked increase in malondialdehyde (MDA)contentand decrease in superoxide dismutase (SOD) activity, but ST had no effect onMDAcontent and SOD activity. Treatment with NO donors caused a reduction of PEG-and DH-induced increase in MDA content and decrease in SOD activity. Theprotective effect of NO donors on promotion of senescence, increase in lipidperoxidation and decrease in SOD activity induced by PEG and DH was reversed by2-(4-carboxy-2-phenyl)-4,4,5,5- tetra-methylinmidazoline-1-oxyl-3-oxide, a NOspecific scavenger, suggesting that the protective effect of NO donors isattributed to NO released. The inhibition of PEG- and DH- enhanced senescenceofrice leaves by NO is most likely mediated through increasing SOD activity anddecrease in lipid peroxidation.