Use of ginger extract and bacterial inoculants for the suppression of Alternaria solani causing early blight disease in Tomato

被引：0

作者：

Sajjad Hyder

Amjad Shahzad Gondal

Anam Sehar

Aimen Razzaq Khan

Nadia Riaz

Zarrin Fatima Rizvi

Rashid Iqbal

Mohamed S. Elshikh

Khaloud M. Alarjani

Muhammed Habib ur Rahman

Muhammad Rizwan

机构：

[1] Government College Women University Sialkot,Department of Botany

[2] Bahauddin Zakariya University,Department of Plant Pathology

[3] Lahore Garrison University Lahore,Directorate of Student Affairs and Student Counselling Service – SA&C

[4] Lahore College for Women University,Department of Botany

[5] The Islamia University of Bahawalpur,Department of Agronomy, Faculty of Agriculture and Environment

[6] King Saud University,Department of Botany and Microbiology, College of Science

[7] University of Bonn,Institute of Crop Science and Resource Conservation (INRES)

[8] MNS-University of Agriculture,Department of Seed Science and Technology, Institute of Plant Breeding and Biotechnology (IPBB)

来源：

BMC Plant Biology | / 24卷

关键词：

Ginger extract; Bacterial inoculants; Early blight disease; Plant growth promotion; Tomato crop;

D O I：

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摘要：

Early blight (EB), caused by Alternaria solani, is a serious problem in tomato production. Plant growth-promoting rhizobacteria promote plant growth and inhibit plant disease. The present study explored the bio-efficacy of synergistic effect of rhizobacterial isolates and ginger powder extract (GPE) against tomato EB disease, singly and in combination. Six fungal isolates from symptomatic tomato plants were identified as A. solani on the basis of morphological features i.e., horizontal septation (6.96 to 7.93 µm), vertical septation (1.50 to 2.22 µm), conidia length (174.2 to 187.6 µm), conidial width (14.09 to 16.52 µm), beak length (93.06 to 102.26 µm), and sporulation. Five of the twenty-three bacterial isolates recovered from tomato rhizosphere soil were nonpathogenic to tomato seedlings and were compatible with each other and with GPE. Out of five isolates tested individually, three isolates (St-149D, Hyd-13Z, and Gb-T23) showed maximum inhibition (56.3%, 48.3%, and 42.0% respectively) against mycelial growth of A. solani. Among combinations, St-149D + GPE had the highest mycelial growth inhibition (76.9%) over the untreated control. Bacterial strains molecularly characterized as Pseudomonas putida, Bacillus subtilis, and Bacillus cereus and were further tested in pot trials through seed bacterization for disease control. Seeds treated with bacterial consortia + GPE had the highest disease suppression percentage (78.1%), followed by St-149D + GPE (72.2%) and Hyd-13Z + GPE (67.5%). Maximum seed germination was obtained in the bacterial consortia + GPE (95.0 ± 2.04) followed by St-149D + GPE (92.5 ± 1.44) and Hyd-13Z + GPE (90.0 ± 2.04) over control (73.8 ± 2.39) and chemical control as standard treatment (90.0 ± 2). Ginger powder extracts also induce the activation of defence-related enzymes (TPC, PO, PPO, PAL, and CAT) activity in tomato plants. These were highly significant in the testing bacterial inoculants against A. solani infection in tomato crops.

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