Effect of hydrogen peroxide on synthesis of proteins during somatic embryogenesis in Lycium barbarum

被引：20

作者：

Kairong C. ^{[1
,2
]}

Ji L. ^{[1
,2
]}

Gengmei X. ^{[1
,2
]}

Jianlong L. ^{[1
,2
]}

Lihong W. ^{[1
,2
]}

Yafu W. ^{[1
,2
]}

机构：

[1] State Key Laboratory of Arid Agroecology, Lanzhou University

[2] Molecular Plant Pathology Laboratory, USDA-ARS, Beltsville

来源：

Plant Cell, Tissue and Organ Culture | 2002年 / 68卷 / 2期

基金：

中国国家自然科学基金;

关键词：

Hydrogen peroxide; In vitro translation; Lycium barbarum L; Somatic embryogenesis; Synthesis of proteins;

D O I：

10.1023/A:1013871500575

中图分类号：

学科分类号：

摘要：

Direct exposure of calluses of Lycium barbarum L. to an auxin-free medium can induce somatic embryogenesis. The effect of hydrogen peroxide on synthesis of proteins during somatic embryogenesis in Lycium barbarum L. was studied. One-dimensional gel electrophoresis showed that new protein was synthesized by embryogenic callus II (in MS+200 μmol 1-1 H2O2 medium). Therefore, we suggested that there was a synthesis of H2O2-induced protein during somatic embryogenesis of Lycium barbarum L. Cycloheximide and actinomycin D prevented the synthesis of the new protein (H2O2-induced) effectively. The results of two-dimensional gel electrophoresis showed that the differences in the protein patterns between embryogenic callus occurred at 35∼55 kDa with a pI-level of about 5∼6. Changes at the transcriptional level in embryogenic callus I and II were analyzed by comparing in vitro translation patterns. The results showed that several new in vitro-translated proteins were found in embryogenic callus treated with hydrogen peroxide. It suggests that the exogenous added H2O2 induced genes expression at the mRNA level during somatic embryogenesis of Lycium barbarum L.

引用

页码：187 / 193

页数：6

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