Chemical Composition and In Vitro Antioxidant Activity of Essential Oil from a Hepatoprotective Herbal Mix

The chemical composition of essential oil (EO) (0.57 – 0.80% yield) from a hepatoprotective herbal mix was analyzed by GC/MS. The major constituents of the EO were 4-epiacorenone (15.04%), dihydroisocalamendiol (11.55%), shyobunone (7.75%), thymol (6.91%), isoshyobunone (3.87%), Z-azarone (3.55%), a-selinene (3.19%), isocalamendiol (2.09%), camphor (1.23%), a-calacorene (1.23%), acoranone isomers 1 (3.07%) and 2 (1.61%), 1,8-cineol (1.61%), spathulenol (1.73%), caryophyllene oxide (1.65%), and caryophyllene (1.01%), which accounted for 67.09% of the oil composition. It was established that thyme, tansy, and absinthe contributed most significantly to the total antioxidant activity of the herbal mix.