Genome-wide SNP discovery, linkage mapping, and analysis of QTL for morpho-physiological traits in rice during vegetative stage under drought stress

被引：0

作者：

Rizky Dwi Satrio

Miftahul Huda Fendiyanto

Ence Darmo Jaya Supena

Sony Suharsono

Miftahudin Miftahudin

机构：

[1] Bogor Agricultural University (IPB University),Plant Biology Graduate Program, Department of Biology, Faculty of Mathematics and Natural Sciences

[2] Bogor Agricultural University (IPB University),Department of Biology, Faculty of Mathematics and Natural Sciences

[3] The Republic of Indonesia Defense University (Unhan RI),Department of Biology, Faculty of Military Mathematics and Natural Sciences

[4] Komplek Indonesia Peace and Security Center (IPSC) Sentul,Faculty of Military Mathematics and Natural Sciences

[5] The Republic of Indonesia Defense University (Unhan RI),undefined

[6] Komplek Indonesia Peace and Security Center (IPSC) Sentul,undefined

来源：

Physiology and Molecular Biology of Plants | 2021年 / 27卷

关键词：

Drought tolerance index; Genotyping-by-sequencing; Leaf gas exchange; Recombinant inbred line; Rice;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Drought tolerance in rice is controlled by several genes and is inherited quantitatively. Low genetic map density and the use of phenotypic traits that do not reflect the corresponding tolerance level have been obstacles in genetic analyses performed to identify genes that control drought-tolerant traits in rice. The current study aimed to construct a genetic map from high-density single-nucleotide polymorphism (SNP) markers generated from genome sequences of recombinant inbred lines (RILs), derived from IR64 × Hawara Bunar. Moreover, it sought to analyze the quantitative trait loci (QTL) and identify the drought tolerance candidate genes. A linkage map along 1980 cM on the 12 rice chromosomes was constructed employing 55,205 SNP markers resulting from the RIL genome sequences. A total of 175 morpho-physiological traits pertaining to drought stress were determined. A total of 41 QTLs were detected in 13 regions on rice chromosomes 1, 3, 6, 8, 9, and 12. Moreover, three hotspot QTL regions were found on chromosomes 6 and 8, along with two major QTL on chromosome 9. Differential gene expression for the loci within the QTL physical map intervals revealed many potential candidate genes. The markers tightly linked to the QTL and their candidate genes can potentially be used for pyramiding in marker-assisted breeding in order to achieve genetic improvement concerning the tolerance of rice to drought stress.

引用

页码：2635 / 2650

页数：15

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