Inoculation of Lupinus luteus with White Root Rot Fungus, Rosellinia necatrix, to Estimate Virulence

被引:12
作者
Yukari UETAKE
Hitoshi NAKAMURA
Masao ARAKAWA
Ikuko OKABE
Naoyuki MATSUMOTO
机构
[1] Bio-oriented Technology Research Advancement Institution,
[2] National Institute for Agro-Environmental Sciences,undefined
[3] Tsukuba 305–8604,undefined
[4] Japan,undefined
[5] National Institute for Agro-Environmental Sciences,undefined
[6] Tsukuba 305–8604,undefined
[7] Japan,undefined
关键词
Key words : Rosellinia necatrix, white root rot, virulence, Lupinus luteus.;
D O I
10.1007/PL00013031
中图分类号
学科分类号
摘要
An inoculation method using Lupinus luteus was developed for estimating virulence of isolates of the white root rot fungus, Rosellinia necatrix Prillieux. Fungal cultures grown on pieces of mulberry twigs were placed in contact with the hypocotyl of 3-week-old seedlings growing in pots of soil. Disease development was uniform and reproducible in repeated experiments. Of 24 isolates with double-stranded RNA, eight were weakly virulent. This method is useful throughout the year for estimating virulence of many isolates of the fungus and for screening for hypovirulent isolates.
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页码:285 / 287
页数:2
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