Genotypical features in the expression of allozymes of β-specific hydrolase of carboxylic esters in wild-type Drosophila melanogaster

被引:0
作者
A. M. Andrievskii
机构
[1] Mechnikov National University,
来源
Cytology and Genetics | 2008年 / 42卷
关键词
Carboxylic Ester; Genotypical Feature; Scanning Pattern; Hybrid Enzyme; Recessive Homozygote;
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摘要
The expression level of electrophoretically separated S- and F-allozymes of β-specific esterase (EC 3.1.1.2) in genotypes of wild-type Drosophila melanogaster (males and females) that are monozygous or heterozygous with respect to the locus β-Est is determined by means of computerized densitometry; α-naphthylacetate, β-naphthylacetate, and α-naphthylpropionate are used as the substrates. The intensity of the expression of the esterase is judged from the quantity of reaction product created as a result of simultaneous azo coupling between naphthol and diazonium in 4, 24, 44, and 64 min incubation times. Reliable differences in the expressions of the S- and F-allozymes as a function of the structure of the β-Est locus of genotypically distinct individuals are established. In all the variant experiments, a higher level of summary activity of the S- and F-allozymes of the β-esterase of the heterozygotes by comparison with the individual activity of the F-and S-allozymes of the corresponding homozygotes was demonstrated, independently of the sex of the Drosophila individual. A comparative estimate of the temporal dynamics of the expression of in vitro allozymes of the dominant homozygotes (β-EstS/β-EstS), heterozygotes (β-EstS/β-EstF), and recessive homozygotes (β-EstF/β-EstF) is performed. Possible mechanisms for the occurrence of heterosis according to the character of expression of S- and F-allozymes of β-esterase on the basis of the theory of biochemical enrichment of heterozygote genotypes are considered.
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页码:391 / 397
页数:6
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