Electrochemical DNA biosensors for label-free breast cancer gene marker detection

被引:0
作者
Mehmet Senel
Muamer Dervisevic
Firdevs Kokkokoğlu
机构
[1] Nanoyasam Nanobiotechnology Company,SANKARA Brain & Biotechnology Research Center
[2] Monash University,Monash Institute of Pharmaceutical Sciences
来源
Analytical and Bioanalytical Chemistry | 2019年 / 411卷
关键词
Electrochemical DNA biosensor; Ferrocene; Poly(amidoamine); Dendrimer; Breast cancer;
D O I
暂无
中图分类号
学科分类号
摘要
We present an electrochemical DNA detection strategy based on self-assembled ferrocene-cored poly(amidoamine) dendrimers for the detection of a gene relevant to breast cancer. The chemisorption of three ferrocene-cored poly(amidoamine) generations and hybridization of single-stranded DNA on a Au electrode were studied by cyclic voltammetry and differential pulse voltammetry. The biosensor demonstrated high sensitivity of 0.13 μA/(ng/ml) in the detection of the target DNA with a linear range of 1.3–20 nM and a detection limit of 0.38 nM. The DNA biosensor also has high selectivity for the target DNA, showing a clear signal difference from a noncomplementary sequence and a single-base-mismatch sequence, which was used as a model of BRAC1 gene mutation. The results shown are highly motivating for exploring DNA biosensing technology in the diagnosis of breast cancer caused by mutation of the BRAC1 gene.
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页码:2925 / 2935
页数:10
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