Negative effect of combined cysteine and glutathione in soy lecithin-based extender on post-thawed ram spermatozoa

被引：0
作者：
Mahdi Zhandi
Mohsen Sharafi
机构：
[1] University of Tehran,Department of Animal Science, College of Agriculture and Natural Resources
[2] Tarbiat Modares University,Department of Poultry Sciences, Faculty of Agriculture
来源：
Cell and Tissue Banking | 2015年 / 16卷
关键词：
Cysteine; Freezing; Glutathione; Ram; Sperm;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This study was conducted to evaluate the effect of combined cysteine and glutathione in soy lecithin-based semen extender on post-thawed ram sperm quality. A total of 28 ejaculates were collected twice a week (from four rams) during breeding season. In each replicate, semen samples (n = 4, one ejaculate for each ram) were pooled and divided into three equal parts, and each part was diluted with one of following extender: (1) soy lecithin-based extender containing no cysteine and no glutathione (C0–G0), (2) soy lecithin-based extender containing cysteine (5 mM) and glutathione (5 mM) (C5–G5), and (3) soy lecithin-based extender containing cysteine (10 mM) and glutathione (10 mM) (C10–G10). After freeze–thawing process, motility and velocity parameters, plasma membrane integrity and functionality, mitochondrial activity, and apoptosis features of spermatozoa were evaluated. The obtained results showed that total and progressive motility, plasma membrane integrity and functionality, and live post-thawed spermatozoa was lower in C10–G10 extender compared to C0–G0 and C5–G5 extenders (P < 0.05). Also, the percentage of dead spermatozoa was higher in C10–G10 extender compared to C0–G0 extender (P < 0.05). Apoptotic spermatozoa was lower in C10–G10 extender compared to C0–G0 and C5–G5 extenders (P < 0.05). All velocity parameters, exception of BCF, did not different between extenders (P > 0.05). In conclusion, it seems that high concentration of combined cysteine and glutathione in soy lecithin-based semen extender has a detrimental effect of post-thawed ram sperm quality.
引用
收藏
页码：443 / 448
页数：5
相关论文
共 137 条
  • [1] Aboagla EME(2004)Effects of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa Theriogenology 62 1160-1172
  • [2] Terada T(1998)Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa Biol Reprod 59 1037-1046
  • [3] Aitken RJ(1983)Taurine, hypotaurine, epinephrine and Albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility Biol Reprod 29 548-555
  • [4] Gordon E(2010)Effect of exogenous glutathione in extender on the freezability of Nili-Ravi buffalo ( Anim Sci Pap Rep 28 235-244
  • [5] Harkiss D(1999)) bull spermatozoa Free Radic Biol Med 26 869-880
  • [6] Twigg JP(2008)Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism Small Rumin Res 77 38-44
  • [7] Milne P(2002)Effects of anti-oxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze–thawing process Theriogenology 57 1025-1033
  • [8] Jennigs Z(2001)Generation of reactive oxygen species by equine neutrophils and their effect on motility of equine spermatozoa Theriogenology 56 275-286
  • [9] Irvine DS(1998)Thiols prevent H Theriogenology 50 699-706
  • [10] Alvarez JG(2011)O Theriogenology 76 342-350
12345678910