Production of Extracellular Isoamylase and Endoglucanase by Entrapped Spores of Rhizopus oryzae PR7

被引：2

作者：

Ghosh B. ^{[1
]}

Karmakar M. ^{[1
]}

Ray R.R. ^{[1
,2
]}

机构：

[1] Microbiology Research Laboratory, Department of Zoology, Molecular Biology & Genetics, Presidency University, 86/1, College Street, Kolkata

[2] Postgraduate Department of Zoology, Bethune College, 181 Bidhan Sarani, Kolkata

来源：

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences | 2015年 / 85卷 / 3期

关键词：

Alginate beads; Endoglucanase; Ionotropic gelation; Isoamylase; Rhizopus oryzae;

D O I：

10.1007/s40011-014-0426-4

中图分类号：

学科分类号：

摘要：

The encapsulation of spores of Rhizopus oryzae PR7 by ionotropic gelation and production of isoamylase and endoglucanase was observed in the present study. The immobilized spores showed maximum production of both the enzymes in beads made up of 9 % of alginate concentration. Maximum production of isoamylase and endoglucanase was found when immobilization of spores involved BaCl2 and CaCl2 respectively. Linear negative correlation was found between isoamylase production and number of spores used for the inoculum, while in case of endoglucanase, production value was more or less independent of the inoculum size. The immobilized spores were reutilized effectively for isoamylase production by recycling of the entrapped spores for four consecutive cycles. © 2014, The National Academy of Sciences, India.

引用

页码：733 / 738

页数：5

共 27 条

[1]

Albany N.Y., Biofuel enzymes market—global industry size, market share, trends, analysis and forecast 2012–2018, In, (2013)

[2]

Ghosh B., Ray R.R., Saccharification of raw native starches by extracellular isoamylase of Rhizopus oryzae, Biotechnology, 9, pp. 224-228, (2010)

[3]

Karmakar M., Ray R.R., Extra cellular endoglucanase production by Rhizopus oryzae in solid and liquid state fermentation of agro wastes, Asian J Biotechnol, 2, pp. 27-36, (2010)

[4]

Kosseva M.R., Immobilization of microbial cells in food fermentation processes, Food Bioprocess Technol, 4, pp. 1089-1118, (2011)

[5]

Guo Y., Lou F., Peng Z.Y., Yuan Z.Y., Kinetics of growth and α-amylase production of immobilized Bacillus subtilis in an airlift bioreactor, Biotechnol Bioeng, 35, pp. 99-102, (1990)

[6]

Ahmed S., Abdel-Fattah A., Production of Bacillus licheniformis ATCC 21415 alkaline protease in batch, repeated batch and continuous culture, Malays J Microbiol, 6, 2, pp. 156-160, (2010)

[7]

Dragomirescu M., Preda G., Vintila T., Vlad-Oros B., Bordean D., Savii C., The effect of immobilization on activity and stability of a protease preparation obtained by an indigenous strain Bacillus licheniformis B 40, Rev Roum Chim, 57, 2, pp. 77-84, (2012)

[8]

Beshay U., Production of alkaline protease by Teredinobacter turnirae cells immobilized in Ca-alginate beads, Afr J Biotechnol 2(3):60–65 doi.http://dx.doi.org/10.4314%2Fajb.v2i3, (2003)

[9]

Karandikar S., Prabhune A., Kalele S.A., Gosavi S.W., Kulkarni S.K., Immobilization of thermo tolerant Kluyveromyces marxianus on silica aerogel for continuous production of invertase syrup, Res J Biotechnol, 1, 2, pp. 16-19, (2006)

[10]

Ray R.R., Jana S.C., Nanda G., β-amalyse production by immobilized cells of Bacillus megaterium B<sub>6</sub>, J Basic Microbiol, 35, pp. 113-116, (1995)

← 1 2 3 →