Demonstration of a novel technique to quantitatively assess inflammatory mediators and cells in rat knee joints

被引：22

作者：

Barton N.J. ^{[1
]}

Stevens D.A. ^{[2
]}

Hughes J.P. ^{[2
]}

Rossi A.G. ^{[3
]}

Chessell I.P. ^{[2
]}

Reeve A.J. ^{[2
]}

McQueen D.S. ^{[1
]}

机构：

[1] Division of Neuroscience, University of Edinburgh, Medical College, Edinburgh, EH8 9JZ

[2] Neurology CEDD, GlaxoSmithKline R and D Ltd., Harlow

[3] MRC Centre for Inflammation Research, Queens Medical Research Institute, University of Edinburgh

来源：

Journal of Inflammation | / 4卷 / 1期

基金：

英国医学研究理事会;

关键词：

Synovial Fluid; Knee Joint; Joint Space; Total Cell Count; Sampling Synovial Fluid;

D O I：

10.1186/1476-9255-4-13

中图分类号：

学科分类号：

摘要：

Background. The inflammation that accompanies the pain and swelling associated with osteo- and rheumatoid arthritis is mediated by complex interactions of inflammatory mediators. Cytokines play a pivotal role in orchestrating many of these processes, including inflammatory cell recruitment, adhesion and activation. In addition, prostaglandins are secreted into the synovial cavity and are involved in perpetuation of local inflammation, vasodilatation and vasoconstriction, and also with bone resorption. Pre-clinical models have been developed in order to correlate to the human disease and principle among these is the adjuvant-induced arthritis model in the rat. Methods. We have developed a technique to quantitatively assess the contents of synovial fluid samples from rat joints. Two needles joined together are inserted into the knee joint of anaesthetised rats and connected to a Watson-Marlow perfusion pump. Sterile saline is infused and withdrawn at 100 μl min -1 until a 250 μl sample is collected. Results. Our results demonstrate up to 125 fold increases in synovial IL1α and IL1β concentrations, approximately 30 fold increases in levels of IL6 and IL10 and a 200-300 fold elevation in synovial concentrations of TNFα during FCA-induced experimental arthritis. Finally, this novel technique has demonstrated a dose-response relationship between FCA and the total cell counts of synovial perfusates. Conclusion. In summary, this new technique provides a robust method for quantifying inflammatory mediators and cells from the synovial cavity itself, thereby detailing the inflammatory processes from within the capsule and excluding those processes occurring in other tissues surrounding the entire articulation. © 2007 Barton et al; licensee BioMed Central Ltd.

引用

共 41 条

[1]

Sweeney S.E., Firestein G.S., Rheumatoid arthritis: Regulation of synovial inflammation, Int J Biochem Cell Biol, 36, pp. 372-378, (2004)

[2]

Kubota E., Kubota T., Matsumoto J., Shibata T., Murakami K.I., Synovial fluid cytokines and proteinases as markers of temporomandibular joint disease, J Oral Maxillofac Surg, 56, pp. 192-198, (1998)

[3]

Alstergren P., Ernberg M., Kvarnstrom M., Kopp S., Interleukin-1beta in synovial fluid from the arthritic temporomandibular joint and its relation to pain, mobility, and anterior open bite, J Oral Maxillofac Surg, 56, (1998)

[4]

Chang H., Israel H., Analysis of inflammatory mediators in temporomandibular joint synovial fluid lavage samples of symptomatic patients and asymptomatic controls, J Oral Maxillofac Surg, 63, pp. 761-765, (2005)

[5]

Rooney M., Symons J.A., Duff G.W., Interleukin 1 beta in synovial fluid is related to local disease activity in rheumatoid arthritis, Rheumatol Int, 10, pp. 217-219, (1990)

[6]

Ulfgren A.K., Grondal L., Lindblad S., Khademi M., Johnell O., Klareskog L., Andersson U., Interindividual and intra-articular variation of proinflammatory cytokines in patients with rheumatoid arthritis: Potential implications for treatment, Ann Rheum Dis, 59, pp. 439-447, (2000)

[7]

Barrera P., Boerbooms A.M., Van De Putte L.B., Van Der Meer J.W., Effects of antirheumatic agents on cytokines, Semin Arthritis Rheum, 25, pp. 234-253, (1996)

[8]

Eastgate J.A., Symons J.A., Wood N.C., Grinlinton F.M., Di Giovine F.S., Duff G.W., Correlation of Plasma Interleukin 1 Levels with Disease Activity in Rheumatoid Arthritis, 2, pp. 706-709, (1988)

[9]

Houssiau F.A., Devogelaer J.P., Van Damme J., De Deuxchaisnes C.N., Van Snick J., Interleukin-6 in synovial fluid and serum of patients with rheumatoid arthritis and other inflammatory arthritides, Arthritis Rheum, 31, pp. 784-788, (1988)

[10]

Christodoulou C., Choy E.H., Joint inflammation and cytokine inhibition in rheumatoid arthritis, Clin Exp Med, 6, pp. 13-19, (2006)

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