Dehalogenation of 4 - Chlorobenzoic acid by pseudomonas isolates

被引：12
作者：
Banta G. [1 ]
Kahlon R.S. [1 ]
机构：
[1] Department of Microbiology, Punjab Agricultural University
来源：
Indian Journal of Microbiology | 2007年 / 47卷 / 2期
关键词：
4-chlorobenzoic acid; Degradation; Dehalogenase; Pseudomonas aeruginosa; Pseudomonas putida;
D O I：
10.1007/s12088-007-0027-5
中图分类号：
学科分类号：
摘要：
Twenty three bacterial isolates either pure or consortium were initially screened on the basis of their ability to degrade as well as dechlorinate 4 - chlorobenzoic acid (4-CBA). Based on comparative growth response, three pure isolates Pseudomonas putida GVS-4, Pseudomonas aeruginosa GVS-18 and Pseudomonas aeruginosa GWS-19 and a consortium SW-2 was finally selected for further studies. The enzyme studies performed with cell free extracts revealed that dehalogenase activity was substrate specific with maximum activity at 300 μgml-1 substrate concentration. Catechol 1,2 dioxygenase activity was found to be present in cell free extracts suggesting that 4 - chlorobenzoic acid (4-CBA) is catabolized by ortho-ring cleavage pathway. The dehalogenase enzyme profile showed single enzyme band in case of GVS-4 (Rm 0.76), GVS-18 (Rm 0.84), GWS -19 (Rm 0.85) and two bands in SW-2 (Rm 0.71 & 0.10). © 2007 Association of Microbiologists of India.
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页码：139 / 143
页数：4
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