A Novel Indirect Competitive Enzyme-Linked Immunosorbent Assay Format for the Simultaneous Determination of Ractopamine and Phenylethanolamine A Residues in Swine Urine

被引：0
作者：
Dapeng Peng
Lan Zhao
Lingyan Zhang
Yuanhu Pan
Yanfei Tao
Yulian Wang
Feng Sheng
Zonghui Yuan
机构：
[1] Huazhong Agricultural University,National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues
[2] Hubei University,Hubei Collaborative Innovation Center for Green Transformation of Bio
来源：
Food Analytical Methods | 2019年 / 12卷
关键词：
Ractopamine; Phenylethanolamine A; Indirect competitive enzyme-linked immunosorbent assay; Monoclonal antibody; Residues;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
For monitoring ractopamine (RAC) and phenylethanolamine A (PA) with high efficiency, a novel indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) format was developed. At first, two specific monoclonal antibodies (mAbs) against RAC and PA were prepared, respectively. Based on the finding that both mAbs RAC and PA could recognize the same coating antigen RAC-SA-OVA, a novel ic-ELISA format was constructed, in which the limit of detection (LOD), recoveries, and coefficient of variation of the ic-ELISA developed for RAC residues were 0.35 μg L−1, 96.7~108.6%, and less than 6.8%, and for PA residues, they were 0.11 μg L−1, 97.8~110.2%, and less than 9.4%, respectively. The developed method also exhibited a positive correlation with the results of HPLC-MS conducted on the samples. These data indicate that the developed novel ic-ELISA is reliable and could be used in a routine application for the simultaneous determination of RAC and PA residues in swine urine.
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页码：1077 / 1085
页数：8
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