Assessment of genetic diversity amongst Ugandan sesame (Sesamum indicum L.) landraces based on agromorphological traits and genetic markers

被引：17

作者：

Sehr E.M. ^{[1
]}

Okello-Anyanga W. ^{[2
,3
]}

Hasel-Hohl K. ^{[1
]}

Burg A. ^{[1
]}

Gaubitzer S. ^{[1
]}

Rubaihayo P.R. ^{[2
]}

Okori P. ^{[2
]}

Vollmann J. ^{[4
]}

Gibson P. ^{[2
,5
]}

Fluch S. ^{[1
]}

机构：

[1] AIT Austrian Institute of Technology, Health & Environment Dept, Konrad-Lorenz Str. 24, Tulln

[2] Department of Agricultural Production, School of Agricultural Sciences, Makerere University, P.O. Box. 7062, Kampala, Ampala

[3] National Semi-Arid Resources Research Institute (NaSARRI), P.O. Soroti, Serere

[4] Department of Crop Sciences, Univ. of Natural Resources and Applied Life Sciences (BOKU), Konrad-Lorenz Str. 20, Tulln

[5] Department of Plant, Soil and Agricultural Systems, Illinois University, Carbondale, IL

来源：

Journal of Crop Science and Biotechnology | 2016年 / 19卷 / 1期

关键词：

Genetic diversity; Landraces; Molecular marker; Sesamum indicum L; SSR;

D O I：

10.1007/s12892-015-0105-x

中图分类号：

学科分类号：

摘要：

Sesame (Sesamum indicum L.) is one of the most important ancient oilseed crops grown throughout the tropical and subtropical regions of the world. In Uganda, most of the cultivated sesame varieties are local landraces which are frequently traded between farmers. Although these traditional landraces are an important source of genetic diversity, knowledge of their genetic diversity is still limited. Agromorphological traits and a set of published and newly developed microsatellite markers were analyzed on a collection of 121 accessions of Ugandan sesame landraces. CpSSR analysis revealed four haplotypes, whereby haplotype B was present in 96% of the individuals. The analysis of nSSR markers from 6 non-coding regions revealed a mean PIC value of 0.56 whereas the PIC value of eight selected EST-derived SSRs was 0.26. Accession-wise, the expected heterozygosity (He) varied from 0 to 0.396. AMOVA revealed that the majority of the variance occurred among the individuals accounting for 75% of the total variation, only 6% was attributed to differences among the districts, pointing towards a high gene flow (Nm = 4.476). These results are supported by the PCoA analysis as well as the NJ tree both of which revealed no clustering of the accessions according to their geographic origin. Also the statistical analysis of 10 agromorphological traits indicated no clear pattern related to the geographic origin. Such a poor grouping, indicative of considerable gene flow across geographic domains, could be explained either by a high outcrossing rate, and/or through extensive seed trading. © Korean Society of Crop Science and Springer 2016.

引用

页码：117 / 129

页数：12

共 36 条

[1]

Agarwal M., Shrivastava N., Padh H., Advances in molecular marker techniques and their applications in plant sciences, Plant Cell Rep., 27, pp. 617-631, (2008)

[2]

Anderson J., Churchill G., Autrique J., Tanksley S., Sorrells M., Optimizing parental selection for genetic linkage maps, Genome, 36, pp. 181-186, (1993)

[3]

Arriel N.H.C., Di Mauro A.O., Arriel E.F., Uneda-Trevisoli S.H., Costa M.M., Barbaro I.M., Muniz F.R.S., Genetic divergence in sesame based on morphological and agronomic traits, Crop Breed. Appl. Biotechnol., pp. 253-261, (2007)

[4]

Bedigian D., Harlan J., Evidence for cultivation of sesame in the ancient world, Econ. Bot., 40, pp. 137-154, (1986)

[5]

Bhattacharyya U., Pandey S.K., Dasgupta T., Identification of EST-SSRs and FDM in sesame (Sesamum indicum L.) through data mining, Sch. J. Agric. Sci., 4, pp. 60-69, (2014)

[6]

Bryan G.J., McNicoll J., Ramsay G., Meyaer R.C., De Jong W.S., Polymorpjic simple sequence repeat markers in chloroplast genomes of Solanaceous plants, Theor. Appl. Gent., 99, pp. 859-867, (1999)

[7]

Dixit A., Jin M.-H., Chung J.-W., Yu J.-W., Chung H.-K., Ma K.H., Park Y.-J., Cho E.-G., Development of polymorphic microsatellite markers in sesame (Sesamum indicum L.), Mol. Ecol. Notes, 5, pp. 736-738, (2005)

[8]

Fluch S., Burg A., Kopecky D., Homolka A., Spiess N., Vendramin G., Characterization of variable EST SSR markers for Norway spruce (Picea abies L.), BMC Res. Notes, 4, (2011)

[9]

Gebremichael D.E., Parzies H.K., Genetic variability among landraces of sesame in Ethiopia, Afr. Crop Sci. J., (2011)

[10]

Hayward A., Tollenaere R., Dalton-Morgan J., Batley J., Molecular Marker Applications in Plants, Plant Genotyping, Methods in Molecular Biology, pp. 13-27, (2015)

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