Following evolutionary paths to protein-protein interactions with high affinity and selectivity

被引：0
作者：
Kalia Bernath Levin
Orly Dym
Shira Albeck
Shlomo Magdassi
Anthony H Keeble
Colin Kleanthous
Dan S Tawfik
机构：
[1] Weizmann Institute of Science,Department of Biological Chemistry
[2] Casali Institute of Applied Chemistry,Department of Biology (Area 10)
[3] The Hebrew University of Jerusalem,undefined
[4] Givat Ram,undefined
[5] Jerusalem,undefined
[6] Israel.,undefined
[7] Israel Structural Proteomics Center,undefined
[8] Weizmann Institute of Science,undefined
[9] University of York,undefined
[10] Present address: Departments of Biochemistry and Infection,undefined
[11] Immunity and Inflammation,undefined
[12] University of Leicester,undefined
[13] Leicester,undefined
[14] UK.,undefined
来源：
Nature Structural & Molecular Biology | 2009年 / 16卷
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学科分类号：
摘要：
Colicins are secreted bacterial toxins. To avoid killing the producer organism, each colicin is coexpressed with a high-affinity inhibitor, or immunity protein (Im). The evolution of Im-Colicin interfaces and the evolvability traits of protein-protein interactions are now examined using in vitro evolution and structural analyses.
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页码：1049 / 1055
页数：6
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共 115 条
[41]  
Bernath K(1978)Protein-protein interactions in colicin E9 DNase-immunity protein complexes. 2. Cognate and noncognate interactions that span the millimolar to femtomolar affinity range Acta Crystallogr. A 34 517-255
[42]  
Magdassi S(1997)Processing of X-ray diffraction data collected in oscillation mode Acta Crystallogr. D Biol. Crystallogr. 53 240-2132
[43]  
Tawfik DS(2004)On the treatment of negative intensity observations Acta Crystallogr. D. Biol. Crystallogr. 60 2126-W383
[44]  
Maté MJ(2007)Refinement of macromolecular structures by the maximum-likelihood method Nucleic Acids Res. 35 W375-undefined
[45]  
Kleanthous C(undefined)Coot: model-building tools for molecular graphics undefined undefined undefined-undefined
[46]  
Li W(undefined)MolProbity: all-atom contacts and structure validation for proteins and nucleic acids undefined undefined undefined-undefined
[47]  
Dennis CA(undefined)undefined undefined undefined undefined-undefined
[48]  
Moore GR(undefined)undefined undefined undefined undefined-undefined
[49]  
James R(undefined)undefined undefined undefined undefined-undefined
[50]  
Kleanthous C(undefined)undefined undefined undefined undefined-undefined
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