Surface display expression of Bacillus licheniformis lipase in Escherichia coli using Lpp’OmpA chimera

被引：0
作者：
Jae-Hyung Jo
Chan-Wook Han
Seung-Hwan Kim
Hyuk-Jin Kwon
Hyune-Hwan Lee
机构：
[1] Hankuk University of Foreign Studies,Department of Bioscience and Biotechnology and Protein Research Center of GRRC, College of Natural Sciences
来源：
Journal of Microbiology | 2014年 / 52卷
关键词：
surface display; lipase; Lpp’OmpA;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The lipase from Bacillus licheniformis ATCC14580 was displayed on the cell surface of Escherichia coli using Lpp’OmpA as the anchoring protein. The expressed Lpp’OmpA-lipase fusion protein has a molecular weight of approximately 35 kDa, which was confirmed by SDS-PAGE and western blot analysis. The Lpp’OmpA-lipase fusion protein was located on the cell surface, as determined by immunofluorescence confocal microscopy and flow cytometry. The enzyme activity of the surface-displayed lipase showed clear halo around the colony. The cell surface-displayed lipase showed the highest activity of 248.12 ± 9.42 U/g (lyophilized cell) at the optimal temperature of 37°C and pH 8.0. The enzyme exhibited the highest activity toward the substrate p-nitrophenyl caprylate (C8). These results suggest that E. coli, which displayed the lipase on its surface, could be used as a whole cell biocatalyst.
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页码：856 / 862
页数：6
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