Evaluation of different methods for immobilization of Candida antarctica lipase B (CalB lipase) in polyurethane foam and its application in the production of geranyl propionate

被引：0
作者：
Gabrieli Nicoletti
Eliane P. Cipolatti
Alexsandra Valério
NatáliaThaisa Gamba Carbonera
Nicole Spillere Soares
Eron Theilacker
Jorge L. Ninow
Débora de Oliveira
机构：
[1] Federal University of Santa Catarina (UFSC),Chemical and Food Engineering Department
来源：
Bioprocess and Biosystems Engineering | 2015年 / 38卷
关键词：
Lipase; Polyurethane foam; Esters; Geranyl propionate;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
With the aim of studying the best method for the interaction of polyurethane (PU) foam and Candida antarctica lipase B, different methods of CalB immobilization were studied: adsorption (PU–ADS), bond (using polyethyleneimine) (PU–PEI), ionic adsorption by PEI with cross-linking with glutaraldehyde (PU–PEI–GA) and entrapment (PU). The characterization of immobilized enzyme derivatives was performed by apparent density and Fourier transform infrared spectroscopy. The free enzyme and enzyme preparations were evaluated at different pH values and temperatures. The highest enzyme activity was obtained using the PU method (5.52 U/g). The methods that stood out to compare the stabilities and kinetic parameters were the PU and PU–ADS. Conversions of 83.5 and 95.9 % for PU and PU–ADS derivatives were obtained, in 24 h reaction, using citronella oil and propionic acid as substrates.
引用
收藏
页码：1739 / 1748
页数：9
相关论文
共 107 条
[1]  
Abbas H(2003)Aroma synthesis by immobilized lipase from Enzyme Microb Technol 32 589-595
[2]  
Comeau L(2009) sp J Mol Catal B Enzym 60 57-63
[3]  
Mahapatra P(2009)Enzymatic synthesis of fruit flavor esters by immobilized lipase from Biochem Eng J 43 327-332
[4]  
Kumari A(2013) optimized with response surface methodology Biochem Eng J 73 72-79
[5]  
Garlapati VK(2008)Synthesis of ethyl butyrate in organic media catalyzed by Langmuir 24 8877-8884
[6]  
Banerjee R(1988) lipase immobilized in polyurethane foams: a kinetic study Enzyme Microb Technol 10 375-382
[7]  
Nag A(2014)Surfactant-coated lipase immobilized in magnetic nanoparticles for multicycle ethyl isovalerate enzymatic production Food Chem 143 319-324
[8]  
Pires-Cabral P(2013)Immobilization of lipases on hydrophobilized zirconia nanoparticles: highly enantioselective and reusable biocatalysts J Mol Catal B Enzym 98 78-86
[9]  
Fonseca MMR(2009)Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes Energy Fuels 23 5725-5730
[10]  
Ferreira-Dias S(2013)Highly efficient solvent-free synthesis of 1,3-diacylglycerols by lipase immobilized on nano-sized magnetite particles J Mol Catal B Enzym 91 59-66
12345678910