Isolation and characterization of polymorphic microsatellite loci from elongate loach (Leptobotia elongate), a threatened fish species endemic to the Yangtze River

被引:0
作者
Hong Yan Liu
Fei Xiong
Xin Bin Duan
Da Qing Chen
机构
[1] Jianghan University,College of Life Science
[2] Chinese Academy of Fishery Sciences,Yangtze River Fisheries Research Institute
来源
Conservation Genetics Resources | 2012年 / 4卷
关键词
Elongate loach; Microsatellite; Polymorphism;
D O I
暂无
中图分类号
学科分类号
摘要
Elongate loach (Leptobotia elongate) is a threatened fish species endemic to the Yangtze River in China. A first set of 22 polymorphism microsatellite loci isolated from this species was characterized. The number of alleles, observed and expected heterozygosity ranged from 2 to 12, from 0.2667 to 1.000 and from 0.2391 to 0.9011, respectively. Seven loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. The polymorphism information content ranged from 0.204 to 0.857. Ten loci were highly informative, 11 were moderately informative, and one was slightly informative. These polymorphic microsatellite markers will be useful for genetic monitoring of natural populations and management of breeding stocks in hatcheries.
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页码:129 / 131
页数:2
相关论文
共 25 条
  • [1] Botstein D(1980)Construction of a genetic linkage map in man using restriction fragment length polymorphisms Am J Human Genet 32 314-331
  • [2] White RL(2005)SSRHunter: development of a local searching software for SSR sites Hereditas 5 808-810
  • [3] Skolnick M(2004)Postembryonic development and growth of larval and juvenile elongate loach Acta Hydrobiol Sin 1 96-100
  • [4] Davis RW(2009) (Bleeker) Environ Biol Fish 85 287-288
  • [5] Li Q(2004)Threatened fishes of the world: Mol Ecol Notes 4 535-538
  • [6] Wan J(2010) (Bleeker 1870) (Botiidae) Environ Biol Fish 87 295-296
  • [7] Liang Y(2002)Micro-checker: software for identifying and correcting genotyping errors in microsatellite data Mol Ecol 11 1-16
  • [8] Hu X(undefined)Threatened fishes of the world: undefined undefined undefined-undefined
  • [9] Yu G(undefined) Bleeker 1870 (Bottiinae) undefined undefined undefined-undefined
  • [10] Huang D(undefined)Strategies for microsatellite isolation: a review undefined undefined undefined-undefined