Assessment of long-term cultivated human precision-cut lung slices as an ex vivo system for evaluation of chronic cytotoxicity and functionality

被引：0

作者：

Vanessa Neuhaus

Dirk Schaudien

Tatiana Golovina

Ulla-Angela Temann

Carolann Thompson

Torsten Lippmann

Claus Bersch

Olaf Pfennig

Danny Jonigk

Peter Braubach

Hans-Gerd Fieguth

Gregor Warnecke

Vidadi Yusibov

Katherina Sewald

Armin Braun

机构：

[1] Fraunhofer Institute for Toxicology and Experimental Medicine,

[2] Hannover,undefined

[3] Germany,undefined

[4] Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH),undefined

[5] Member of the German Centre for Lung Research (DZL),undefined

[6] Member of the REBIRTH Cluster of Excellence,undefined

[7] Fraunhofer USA Center for Molecular Biotechnology,undefined

[8] Institute for Pathology,undefined

[9] Hannover Medical School,undefined

[10] Hanover,undefined

[11] Germany,undefined

[12] Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH),undefined

[13] Member of the German Centre for Lung Research (DZL),undefined

[14] Klinikum Region Hannover (KRH),undefined

[15] Institute of Pathology,undefined

[16] Klinikum Region Hannover (KRH),undefined

[17] Division of Thoracic and Vascular surgery,undefined

[18] Division of Cardiac,undefined

[19] Thoracic,undefined

[20] Transplantation,undefined

[21] and Vascular Surgery,undefined

[22] Hannover Medical School,undefined

[23] Hanover,undefined

[24] Germany,undefined

[25] Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH),undefined

[26] Member of the German Centre for Lung Research (DZL),undefined

[27] Institute of Immunology,undefined

[28] Hannover Medical School,undefined

来源：

Journal of Occupational Medicine and Toxicology | / 12卷

关键词：

Precision-cut lung slices; PCLS; Long-term cultivation; Bronchoconstriction; Human; Cytokines; Cytotoxicity;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

引用

共 40 条

[1] Schleputz M(2012)Neurally mediated airway constriction in human and other species: a comparative study using precision-cut lung slices (PCLS) PLoS One 7 e47344-249
[2] Rieg AD(2014)Milrinone relaxes pulmonary veins in Guinea pigs and humans PLoS One 9 e87685-358
[3] Seehase S(1995)Maintaining intact mature lung tissue in culture using low melt agarose Methods Cell Sci 17 245-undefined
[4] Spillner J(2017)Evaluation of inflammatory and immune responses in long-term cultured human precision-cut lung slices Hum Vaccin Immunother 13 351-undefined
[5] Perez-Bouza A(2016)Tissue turnover of collagen type I, III and elastin is elevated in the PCLS model of IPF and can be restored back to vehicle levels using a phosphodiesterase inhibitor Respir Res 17 76-undefined
[6] Braunschweig T(2014)Innate immune response to a H3N2 subtype swine influenza virus in newborn porcine trachea cells, alveolar macrophages, and precision-cut lung slices Vet Res 45 42-undefined
[7] Rieg AD(2012)LPS-induced lung inflammation in marmoset monkeys - an acute model for anti-inflammatory drug testing PLoS One 7 e43709-undefined
[8] Suleiman S(undefined)undefined undefined undefined undefined-undefined
[9] Perez-Bouza A(undefined)undefined undefined undefined undefined-undefined
[10] Braunschweig T(undefined)undefined undefined undefined undefined-undefined

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