Characterization of p-hydroxybenzaldehyde dehydrogenase, the final enzyme of p-hydroxybenzoic acid biosynthesis in hairy roots of Daucus carota

被引：0
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作者：
Debabrata Sircar
Chiranjit Mukherjee
Till Beuerle
Ludger Beerhues
Adinpunya Mitra
机构：
[1] Indian Institute of Technology Kharagpur,Natural Product Biotechnology Group, Agricultural and Food Engineering Department
[2] Technische Universität Braunschweig,Institute of Pharmaceutical Biology
来源：
Acta Physiologiae Plantarum | 2011年 / 33卷
关键词：
-Hydroxybenzaldehyde dehydrogenase; -Hydroxybenzoic acid biosynthesis;
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摘要：
Hairy root cultures of Daucus carota respond to methyl-jasmonate treatment with enhanced accumulation of p-hydroxybenzoic acid. The final C1-side chain of this compound is shaped by p-hydroxybenzaldehyde dehydrogenase (HBD) that catalyzes the formation of p-hydroxybenzoic acid from p-hydroxybenzaldehyde in the presence of NAD+. HBD was biochemically characterized from cell-free hairy root extracts of D. carota. The preferred substrate for HBD was p-hydroxybenzaldehyde. The apparent Km values were 54.8 and 74.4 μM for p-hydroxybenzaldehyde and NAD+, respectively. Divalent metal cations did not significantly affect enzyme activity.
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页码：2019 / 2024
页数：5
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