Local substitution of GDF-15 improves axonal and sensory recovery after peripheral nerve injury

被引:0
作者
Leonore Mensching
Ann-Kathrin Börger
Xialong Wang
Petar Charalambous
Klaus Unsicker
Kirsten Haastert-Talini
机构
[1] Hannover Medical School,Institute of Neuroanatomy
[2] University of Freiburg,Department of Molecular Embryology
[3] Center for Systems Neuroscience (ZSN),undefined
来源
Cell and Tissue Research | 2012年 / 350卷
关键词
GDF-15; Peripheral nerve regeneration; Functional recovery; Nerve morphometry; Epineurial pouch technique;
D O I
暂无
中图分类号
学科分类号
摘要
The growth/differentiation factor-15, GDF-15, has been found to be secreted by Schwann cells in the lesioned peripheral nervous system. To investigate whether GDF-15 plays a role in peripheral nerve regeneration, we substituted exogenous GDF-15 into 10-mm sciatic nerve gaps in adult rats and compared functional and morphological regeneration to a vehicle control group. Over a period of 11 weeks, multiple functional assessments, including evaluation of pinch reflexes, the Static Sciatic Index and of electrophysiological parameters, were performed. Regenerated nerves were then morphometrically analyzed for the number and quality of regenerated myelinated axons. Substitution of GDF-15 significantly accelerated sensory recovery while the effects on motor recovery were less strong. Although the number of regenerated myelinated axons was significantly reduced after GDF-15 treatment, the regenerated axons displayed advanced maturation corroborating the results of the functional assessments. Our results suggest that GDF-15 is involved in the complex orchestration of peripheral nerve regeneration after lesion.
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页码:225 / 238
页数:13
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