Production of Haploids and Doubled Haploids of the Amphiploids Aegilops Variabilis × Secale Cereale

被引：0
作者：
Aleksandra Ponitka
Aurelia Ślusarkiewicz-Jarzina
Barbara Wojciechowska
机构：
[1] Polish Academy of Sciences,Institute of Plant Genetics
来源：
Cereal Research Communications | 2002年 / 30卷
关键词：
anther culture; amphiploids; doubled haploids; haploids; androgenetic embryos;
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摘要：
Anther culture was used to produce haploid plants from Aegilops variabilis ssp. euvariabilis × Secale cereale (inbred line) amphiploids (2n=42) and their parental forms. The anthers were cultured on modified induction media - C17 and PII. Androgenetic embryos were obtained only from amphiploid plants and the frequency depended on genotypes. On C17 medium 20.0–65.2 and on PII medium 1.4–15.7 androgenetic embryos /100 anthers were produced. The embryos were transferred to a regeneration medium 190–2 and 0.1–13.4 of green plants /100 androgenetic embryos were obtained. The haploid (n=21) chromosome number was found in all plants. Doubled haploids were produced by haploid colchicine treatment.
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页码：39 / 45
页数：6
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