Label-free detection repeatability of protein microarrays by oblique-incidence reflectivity difference method

被引：0
作者：
Jun Dai
Lin Li
JingYi Wang
LiPing He
HuiBin Lu
KangCheng Ruan
KuiJuan Jin
GuoZhen Yang
机构：
[1] Chinese Academy of Sciences,Beijing National Laboratory for Condensed Matter Physics, Institute of Physics
[2] Chinese Academy of Sciences,State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences
来源：
Science China Physics, Mechanics and Astronomy | 2012年 / 55卷
关键词：
oblique-incidence reflectivity difference (OIRD); protein microarrays; specificity; repeatability;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We examine the repeatabilities of oblique-incidence reflectivity difference (OIRD) method for label-free detecting biological molecular interaction using protein microarrays. The experimental results show that the repeatabilities are the same in a given microarray or microarray-microarray and are consistent, indicating that OIRD is a promising label-free detection technique for biological microarrays.
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页码：2347 / 2350
页数：3
相关论文
共 48 条
  • [1] Zhu H.(2001)Global analysis of protein activities using proteome chips Science 293 2101-2105
  • [2] Bilgin M.(2004)Protein microarray detection strategies: Focus on direct detection technologies J Immunol Methods 290 121-133
  • [3] Bangham R.(2011)Utilization of unmodified gold nanoparticles in colorimetric detection Sci China-Phys Mech Astron 54 1757-1765
  • [4] Espina V.(2006)Label-free detection methods for protein microarrays Proteomics 6 5493-5503
  • [5] Woodhouse E. C.(2010)Label-free detection techniques for protein microarrays: Prospects, merits and challenges Proteomics 10 731-748
  • [6] Wulfkuhle J.(2007)Oblique-incidence reflectivity difference microscope for label-free high-throughput detection of biochemical reactions in a microarray format Appl Opt 46 1890-1895
  • [7] He S.(2008)A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays Rev Sci Instrum 79 013708-1437
  • [8] Liu D. B.(2010)Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method J Appl Phys 107 063109-1233
  • [9] Wang Z.(2010)Label-free and high-throughput detection of protein microarrays by oblique-incidence reflectivity difference method Chin Phys Lett 27 107801-309
  • [10] Yu X. B.(2010)Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method J Opt 12 095301-1588
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