Culture of limbal stem cells on human amniotic membrane

被引：0
作者：
Mª Esther Rendal-Vázquez
Anahí San-Luis-Verdes
Mª Teresa Yebra-Pimentel-Vilar
Isabel López-Rodríguez
Nieves Domenech-García
Candido Andión-Núñez
Francisco Blanco-García
机构：
[1] Complejo Hospitalario Universitario A Coruña,Unit of Cryobiology, Carretera del Pasaje s/n
[2] Complejo Hospitalario Universitario A Coruña,Anatomopathologic Service, Carretera del Pasaje s/n
[3] Complejo Hospitalario Universitario A Coruña,Service of Ophtalmology, Carretera del Pasaje s/n
[4] Complejo Hospitalario Universitario A Coruña,Biobank, Carretera del Pasaje s/n
[5] Complejo Hospitalario Universitario A Coruña,INIBIC, Carretera del Pasaje s/n
来源：
Cell and Tissue Banking | 2012年 / 13卷
关键词：
Limbal stem cells; p63; ABCG2; Amniotic membrane;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Limbal stem cells (LSC) have an important role in the maintenance of the corneal surface epithelium, and autologous cultured limbal epithelial cell (HLECs) transplantations have contributed substantially to the treatment of the visually disabling condition known as LSC deficiency. A major challenge is the ability to identify LSC in vitro and in situ, and one of the major controversies in the field relates to reliable LSC markers. This study was carried out to evaluate the culture of a limbal biopsy on human amniotic membrane (HAM): directly on the chorionic side and on intact epithelium, and the expression of the stem cell associated markers: ABCG2, p63. HAM has been extensively used for ocular surface reconstruction and has properties which facilitate the growth of epithelial cells controlling inflammation and scarring.
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页码：513 / 519
页数：6
相关论文
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