Toxicological assessment of magnesium oxide nanoparticles in HT29 intestinal cells

被引：0
作者：
Anna Mittag
Thomas Schneider
Martin Westermann
Michael Glei
机构：
[1] Friedrich Schiller University Jena,Department of Nutritional Toxicology, Institute of Nutritional Sciences
[2] Friedrich Schiller University Jena,Electron Microscopy Centre
来源：
Archives of Toxicology | 2019年 / 93卷
关键词：
Flow cytometry; HT29 cells; Magnesium oxide; Nanoparticles; Nanotoxicology; Oral exposure;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Nanoparticles (NPs) are increasingly used in different consumer-related areas, for instance in food packaging or as additives, because of their enormous potential. Magnesium oxide (MgO) is an EU-approved food additive (E number 530). It is commonly used as a drying agent for powdered foods, for colour retention or as a food supplement. There are no consistent results regarding the effects of oral MgO NP uptake. Consequently, the aim of this study was to examine the effects of MgO NPs in the HT29 intestinal cell line. MgO NP concentrations ranged from 0.001 to 100 μg/ml and incubation times were up to 24 h. The cytotoxic and genotoxic potential were investigated. Apoptotic processes and cell cycle changes were analysed by flow cytometry. Finally, oxidative stress was examined. Transmission electron microscopy indicated that there was no cellular uptake. MgO NPs had no cytotoxic or genotoxic effects in HT29 cells and they did not induce apoptotic processes, cell cycle changes or oxidative stress.
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页码：1491 / 1500
页数：9
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