Comparison of Two PCR Systems for the Rapid Detection of Leptospira spp. from Kidney Tissue

被引:0
作者
Anne Mayer-Scholl
Angelika Draeger
Enno Luge
Rainer Ulrich
Karsten Nöckler
机构
[1] Federal Institute for Risk Assessment (BfR),
[2] Friedrich-Loeffler-Institut (FLI),undefined
[3] Institute for Novel and Emerging Infectious Diseases,undefined
来源
Current Microbiology | 2011年 / 62卷
关键词
Diagnostics; PCR; Leptospira;
D O I
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中图分类号
学科分类号
摘要
In this study we compared two routine PCR systems for the detection of Leptospira spp. and assessed their performance when directly applied to kidney samples from small mammals. Although the kappa value of 0.9 indicated a high level of agreement between the tests, the outer membrane lipoprotein gene lipl32 based PCR was more robust and showed a higher number of positive kidney samples.
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页码:1104 / 1106
页数:2
相关论文
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