Evaluation of in vitro chemosensitivity of antitumor drugs using the MTT assay in fresh human breast cancer

被引:0
作者
Jian-Ming Xu
San-Tai Song
Zhong-Ming Tang
Xiao-Qing Liu
Zei-Fei Jiang
Li Zhou
Yan-Bo Li
Yan Huang
机构
来源
Breast Cancer Research and Treatment | 1998年 / 49卷
关键词
in vitro chemosensitivity; human breast cancer chemotherapy; MTT assay;
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摘要
Practical criteria were developed in this paper for the purpose of evaluating chemosensitivity of fresh human breast cancer by the MTT assay. The survival rates at maximum inhibition (Imax %) and the concentrations of drugs which caused fifty percent reduction in absorbance compared to baseline values (IC50) of 175 samples of 10 anti-tumor drugs were evaluated by logistic analyses of the dose-response curves. Distributions of Imax% appeared as normal curves, while those of the IC50 significantly deviated from normal distribution (p < 0.0001). We assessed the in vitro chemosensitivity by comparing the Imax % of each drug on individual samples with the mean Imax % + SD which was obtained from the Imax% of 175 samples. If the individual Imax % > mean Imax % + SD, we thought the tumor sample was resistant to this drug. If the Imax % ≤ mean Imax % + SD, we would compare its IC50 with Q50 which was used as a cutoff point for in vitro chemosensitivity of anti-tumor drugs. The in vitro chemosensitivity could be graded as sensitive (Q1–Q25), intermediate (Q26–Q75), and resistant (Q76–Q100) by means of percentile method. If the individual IC50 ≥ Q76, the tumor sample would be defined as resistant. If the individual IC50 ≤ Q25, it would be defined as sensitive. In the range of Q26–Q75, we used Q50 as a cutoff point between relative sensitivity and relative resistance. Preliminary results showed that the in vitro chemosensitivity to different anti-tumor drugs determined by these criteria were consistent with the clinical response in 83 advanced breast cancer patients.
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页码:251 / 259
页数:8
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